Human SCF ELISA Kit

用户评价
货号-规格
价格
Qty.
CEA-C078-96tests
¥2940.00
CEA-C078-480tests (96tests X 5)
¥13230.00
合计0件 产品金额¥ 0

产品详情

  • 产品概述(Product Overview)

    Human SCF ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human SCF that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:

    1. Standards to calibrate with NIBSC/WHO standards for comparable results.
    2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

  • 产品参数(Product Specifications)

    Assay Type
    Sandwich-ELISA
    Analyte
    SCF
    Format
    96-wells plate breakable into 12 x 8 wells strips
    Reactivity
    Human
    Sensitivity
    15.625 pg/mL
    Assay Time
    3 hr 15 min
    Sample volume
    50 μL
    Range
    31.25 pg/mL-2000 pg/Ml
    Sample Type
    Cell Culture Supernatants, Plasma, Serum.
    NIBSC Code
    91/682

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • 应用说明(Application)

    The kit is developed for quantitative detection of natural and recombinant human SCF in serum, plasma and cell culture supernatants.

    It is for research use only.

  • 流程图(Workflow)

    SCF Workflow

  • 存储(Storage)

    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • 组分(Materials Provided)

    ID
    Components
    Size
    CEA078-C01
    Pre-coated Anti- SCF Antibody Microplate
    1 plate
    CEA078-C02
    Human SCF Standard
    20 μg×2
    CEA078-C03
    Biotin-Anti-SCF Antibody Con. Solution
    200 μL
    CEA078-C04
    Biotin-Antibody Dilution Buffer
    8 mL
    CEA078-C05
    Streptavidin-HRP Con. Solution
    500 μL
    CEA078-C06
    Streptavidin-HRP Dilution Buffer
    15 mL
    CEA078-C07
    20×Washing Buffer
    50 mL
    CEA078-C08
    Sample Dilution Buffer
    15 mL×2
    CEA078-C09
    Substrate Solution
    12 mL
    CEA078-C10
    Stop Solution
    6 mL
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

推荐产品

数据展示

  • 典型数据-Typical Data

    Please refer to DS document for the assay protocol.

     SCF TYPICAL DATA

    For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of SCF is less than 15.625 pg/mL.

验证
  • 样本值(Sample Values)

    Serum Sample: Eight healthy serum samples were evaluated for the concentrations of human SCF in assay.

     SCF SAMPLE VALUES
  • 批内差异(Intra-Assay Statistics)

    Ten replicates of each of four samples containing different SCF concentrations were tested in one assay. Acceptable criteria: CV<10%.

     SCF INTRA-ASSAY STATISTICS
  • 批间差异(Inter-Assay Statistics)

    Five samples containing different concentrations of SCF were tested in independent assays. Acceptable criteria: CV<15%.

     SCF INTER-ASSAY STATISTICS
  • 回收率(Recovery)

    Recombinant SCF was spiked into three human serum samples, and then analyzed. The average recovery of SCF for serum samples is 103.2%.

     SCF RECOVERY

用户评价
发表评论

前沿进展

 
联系我们
项目合作
查看更多项目
实验案例分析
查看更多案例分析
  • 产品基础信息
  • 产品详情
  • 推荐产品
  • 数据展示
  • 用户评价