产品详情
组分(Materials Provided)
IDComponentsSizeRAS224-C01Pre-coated Anti-NA (Influenza A (H3N2)) Antibody Microplate1 plate (8×12 strips)RAS224-C02NA (Influenza A (H3N2)) Standard20 μgRAS224-C03HRP-Anti-NA (Influenza A (H3N2)) Antibody20 μgRAS224-C0410×Washing Buffer50 mLRAS224-C05Dilution Buffer50 mLRAS224-C06Substrate Solution12 mLRAS224-C07Stop Solution7 mL产品概述(Product Overview)
Influenza A (H3N2) Viruses Neuraminidase (NA) Specific ELISA Kit is designed for quantitative detection of antigen and quality control of vaccines.It is based on sandwich-ELISA technology platform to realize the quantitative detection of NA protein in samples.
应用说明(Application)
This kit is developed for specific quantitative detection of Influenza A (H3N2) viruses Neuraminidase (NA) in samples.
It is for research use only.
重构方法(Reconstitution)
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储(Storage)
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理(Assay Principles)
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Influenza A (H3N2) viruses Neuraminidase (NA) protein. The kit consists of Pre-coated Anti-NA (Influenza A (H3N2)) Antibody Microplate and NA (Influenza A (H3N2)) Standard,HRP-Anti-NA (Influenza A (H3N2)) Antibody and buffers.
Your experiment will include 5 simple steps:
a) Bring all reagents and samples to room temperature (20℃-25℃) before use.
b) Add your sample to the plate, take the NA (Influenza A (H3N2)) as Control sample. The samples and Control sample are diluted by Dilution Buffer.
c) Add the HRP-Anti-NA (H3N2) Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add TMB.
e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
质量管理控制体系(QMS)
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数据展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.

For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following ex-ample data is for reference only.

The kit has been tested to specifically identify Influenza A (A/Croatia/10136RV/2023) & (A/District of Columbia/27/2023) NA(H3N2), Influenza A [A/Massachusetts/18/2022 (H3N2)] NA, Influenza A [A/Thailand/8/2022 (H3N2)] NA, Influenza A [A/Darwin/9/2021 (H3N2)] NA, In-fluenza A [Darwin/6/2021 (H3N2)] NA, and do not identify Influenza A [A/Victoria/4897/2022(H1N1)] NA, Influenza A [Wisconsin/67/2022(H1N1)] NA, Influenza B [Austria/1359417/2021] NA and Influenza B [PHUKET/3073/2013] NA.
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