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产品概述（Product Overview）
Human Soluble Mesothelin ELISA Kit, PRO is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human Mesothelin (MSLN) that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
产品参数（Product Specifications）
Assay TypeSandwich-ELISAAnalyteSoluble MesothelinFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity70.0 pg/mLAssay Time2 hr 45 minSample volume50 μLRange156.25 pg/mL-10000 pg/mLSample TypeCell Culture Supernatants, Plasma, Serum.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
应用说明（Application）
The kit is developed for quantitative detection of natural and recombinant human Mesothelin (MSLN) in serum, plasma and cell culture supernatants.
It is for research use only.
流程图（Workflow）
存储（Storage）
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
组分（Materials Provided）
IDComponentsSizeCEA030-C01Pre-coated Anti-Soluble Mesothelin Antibody Microplate1 plateCEA030-C02Human Soluble Mesothelin Standard20 μg×2CEA030-C03Biotin-Anti-Soluble Mesothelin Antibody Con. Solution100 μLCEA030-C04Biotin-Antibody Dilution Buffer8 mLCEA030-C05Streptavidin-HRP Con. Solution500 μLCEA030-C06Streptavidin-HRP Dilution Buffer15 mLCEA030-C0720×Washing Buffer50 mLCEA030-C082×Sample Dilution Buffer15 mL×2CEA030-C09Substrate Solution12 mLCEA030-C10Stop Solution6 mL质量管理控制体系（QMS）
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数据展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of Soluble Mesothelin is less than 70.0 pg/mL.
样本值（Sample Values）
Serum Sample: Thirty-two healthy serum samples were evaluated for the concentrations of human Soluble Mesothelin in assay.
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