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产品参数（Product Specifications）
Assay TypeSandwich-ELISAAnalyteLaminin 521Format96T (8×12 strips)ReactivityHumanRegulatory StatusRUOSensitivity＜25 pg/mLStandard Curve Range25 pg/mL-800 pg/mLAssay Time3 hr 20 minSuitable Sample TypeFor the detection and quantitative determination of Laminin 521 in human serum and cell culture supernates.Sample volume100 μL产品概述（Product Overview）
resDetect™ Human Laminin 521 ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine Human Laminin 521 residues in cell culture supernatants, serum, and plasma. The kit contains GMP human Laminin 521 (ACROBiosystems, cat# GMP-LA5H24) to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of Human Laminin 521.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
组分（Materials Provided）
IDComponentsSizeRES040-C01Pre-coated Anti-Laminin 521 Antibody Microplate1 plate (8×12 strips)RES040-C02Human Laminin 521 Standard20 μgRES040-C03Biotin-Anti-Laminin Antibody20 μgRES040-C04Streptavidin-HRP50 μLRES040-C0510×Washing Buffer50 mLRES040-C062×Dilution Buffer50 mLRES040-C07Substrate Solution12 mLRES040-C08Stop Solution7 mL质量管理控制体系（QMS）
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数据展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
稀释线性（Dilution Linearity）
To assess the linearity of the assay, samples spiked with high concentrations of Laminin 521 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
批内差异（Intra-Assay Statistics）
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.
批间差异（Inter-Assay Statistics）
Three samples of known concentration were tested in three separate assays to assess inter-assay precision.
回收率（Recovery）
Three Laminin 521 with different concentrations were tested to calculate the recovery rate.
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