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产品概述（Product Overview）
Mouse IL-6 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant mouse IL-6 that is present in biological samples, such as serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Ready-to-use reagents to save assay time.
产品参数（Product Specifications）
Assay TypeSandwich-ELISAAnalyteMouse IL-6Format96-wells plate breakable into 12 x 8 wells stripsReactivityMouseSensitivity10 pg/mLRange18.75 pg/mL-600 pg/mLAssay Time2.75 hrSample volume50 μLSample TypeFor the quantitative determination of mouse IL-6 in serum, plasma and cell supernatant.NIBSC Code93/730, NIBSC/WHO (93/730) approximate value (U/mL) = 0.25 x ACRO Mouse IL-6 (pg/mL)Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
应用说明（Application）
The kit is developed for quantitative detection of natural and recombinant mouse IL-6 in serum, plasma and cell culture supernatants.
It is for research use only.
流程图（Workflow）
存储（Storage）
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
组分（Materials Provided）
IDComponentsSizeCEA254-C01Pre-coated Anti-mouse-IL-6 Antibody Microplate1 plateCEA254-C02Mouse IL-6 Standard6 ng×2CEA254-C03Biotin-Anti-mouse-IL-6 Antibody2 μgCEA254-C04Biotin-Antibody Dilution Buffer8 mLCEA254-C05Streptavidin-HRP Con. Solution500 μLCEA254-C06Streptavidin-HRP Dilution Buffer15 mLCEA254-C0720×Washing Buffer50 mLCEA254-C08Sample Dilution Buffer15 mL×2CEA254-C09Substrate Solution12 mLCEA254-C10Stop Solution6 mL质量管理控制体系（QMS）
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数据展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable con-centration of mouse IL-6 is less than 10 pg/mL.
样本值（Sample Values）
Serum Sample: The mice in the experimental group were injected with 15 μg or 30 μg of LPS and bled 4 hours after injection. In contrast, those in the control group received an equivalent volume of normal saline (NS). Serum samples were collected from all mice, and IL-6 concentrations were quantitatively measured using standardized immunoassays.
批内差异（Intra-Assay Statistics）
Ten replicates of each of three samples containing different Mouse IL-6 concen-trations were tested in one assay. Acceptable criteria: CV＜10%.
批间差异（Inter-Assay Statistics）
Three samples containing different concentrations of Mouse IL-6 were tested in independent assays. Acceptable criteria: CV<15%.
回收率（Recovery）
Recombinant IL-6 was spiked into three mouse serum samples, and then analyzed. The average recovery of IL-6 for serum samples is 88.7%.
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