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产品概述（Product Overview）
Mouse IFN-gamma ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant mouse IFN-gamma that is present in biological samples, such as serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Ready-to-use reagents to save assay time.
产品参数（Product Specifications）
Assay TypeSandwich-ELISAAnalyteMouse IFN-gammaFormat96-wells plate breakable into 12 x 8 wells stripsReactivityMouseSensitivity3 pg/mLAssay Time2.75 hrSample volume50 μLRange9.4 pg/mL-600 pg/mLSample TypeCell Culture Supernatants, Plasma, Serum.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
应用说明（Application）
The kit is developed for quantitative detection of natural and recombinant mouse IFN-gamma in serum, plasma and cell culture supernatants.
It is for research use only.
流程图（Workflow）
存储（Storage）
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
组分（Materials Provided）
IDComponentsSizeCEA251-C01Pre-coated Anti-mouse IFN-gamma Antibody Microplate1 plate (8×12 strips)CEA251-C02Mouse IFN-gamma Standard6 ng×2CEA251-C03Biotin-Anti-mouse IFN-gamma Antibody Con. Solution100μLCEA251-C04Biotin-Antibody Dilution Buffer8 mLCEA251-C05Streptavidin-HRP Con. Solution500 μLCEA251-C06Streptavidin-HRP Dilution Buffer15 mLCEA251-C0720×Washing Buffer50 mLCEA251-C08Sample Dilution Buffer15 mL×2CEA251-C09Substrate Solution12 mLCEA251-C10Stop Solution6 mL质量管理控制体系（QMS）
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数据展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable con-centration of mouse IFN-gamma is less than 3 pg/mL.
批内差异（Intra-Assay Statistics）
Ten replicates of each of three samples containing different mouse IFN-gamma concentrations were tested in one assay. Acceptable criteria: CV＜10%.
批间差异（Inter-Assay Statistics）
Three samples containing different concentrations of mouse IFN-gamma were tested in independent assays. Acceptable criteria: CV<15%.
回收率（Recovery）
Recombinant mouse IFN-gamma was spiked into three mouse serum samples, and then analyzed. The average recovery of IFN-gamma for serum samples is 100.2%.
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