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产品参数（Product Specifications）
Assay TypeFACSAnalyteIFN-γFormat100 testsReactivityMouseRegulatory StatusRUOSensitivity1.53 pg/mLStandard Curve Range9.8-10000pg/mLAssay Time2.5 hrSuitable Sample TypeMouse serumSample volume30 μL产品概述（Product Overview）
Multiplex Bead Assay platform allows flow cytometry based on beads with different size and color to quantity multiple soluble cell release factor from a single sample. The performance of this platform has been optimized for specific analysis in cell culture supernatants, plasma and serum samples. Compared to traditional single-analyte methods, it offers significant advantages, including lower sample volume requirements, simpler operation, more broad dynamic range and so on.
We offer a range of individual reagents for each factor, giving you the flexibility to select and combine them according to your specific research requirements.
应用说明（Application）
This Panel (Flow Cytometry Bead Assay) gives quantitative results of Interferon-gamma (IFN-γ) with a sample testing. The performance of this panel has been optimized for specific analysis of cytokines.
It is for research use only.
存储（Storage）
1. Unopened buffer should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The shelf life is 30 days from the date of opening.
组分（Materials Provided）
IDComponentsSizeFMBc001-02Anti-mouse IFN-γ Antibody-coupled APC-beads-B0150 tests×2FABr018-BBiotinylated Monoclonal Anti-Mouse IFN-γ Antibody, Rat IgG1 (AS545)0.25 mL×2IL2-M51P3Mouse Standard Mix-015 μg×2STN-NP119Streptavidin Protein-PE100 μgFMBf001-02APC Positive Control-010.5 mLFMBf001-01PE Positive Control-010.5 mLFCMP-0296-well Sealing film2 piecesFCMP-0196-Well V-Bottom Assay Plate1 plateFCMB-03Assay Buffer-0150 mL×2FCMB-04Wash Buffer-0150 mL×2质量管理控制体系（QMS）
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数据展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
A double logarithmic fitting curve was plotted for IFN-γ. The present curve of each cytokines was ranging from 9.8 pg/mL to 10000 pg/mL.
活性（Bioactivity）-FCM
Please refer to DS document for the assay protocol.
A representative scatter plot showing single bead population in P1 gate (left), the beads conjugated with analyte-sepcific antibody differntiated by APC fluorescence intensity (middle), and fluorescence signal in propotion to concentration of the analyte was measured for each cytokine in PE channel (right). Data was generated on BD FACSymphony™ A1 Flow Cytometer, equipped with blue laser (561 nm) and red laser (637 nm).
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