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产品概述（Product Overview）
Human Complement C5 ELISA Kit ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantify natural and recombinant human Complement C5 (not C5a) that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
产品参数（Product Specifications）
Assay TypeSandwich-ELISAAnalyteComplement C5Format96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity40 pg/mLAssay Time2.75 hrSample volume50 μLRange78.125 pg/mL-5000 pg/mLSample TypeFor the quantitative determination of human Complement C5 in serum, plasma and cell supernatant.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
应用说明（Application）
The kit is developed for quantitative detection of human complement C5 in serum, plasma and cell culture supernates.
It is for research use only.
流程图（Workflow）
存储（Storage）
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
组分（Materials Provided）
IDComponentsSizeCEA241-C01Pre-coated Anti-Complement C5 Antibody Microplate1 plateCEA241-C02Human Complement C5 Standard1 μg×2CEA241-C03Biotin-Anti-Complement C5 Antibody Con. Solution100 μLCEA241-C04Biotin-Antibody Dilution Buffer8 mLCEA241-C05Streptavidin-HRP Con. Solution500 μLCEA241-C06Streptavidin-HRP Dilution Buffer15 mLCEA241-C0720× Washing Buffer50 mLCEA241-C08Sample Dilution Buffer15 mL×2CEA241-C09Substrate Solution12 mLCEA241-C10Stop Solution6 mL质量管理控制体系（QMS）
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数据展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of Complement C5 is less than 40 pg/mL.
样本值（Sample Values）
Twenty-four healthy serum samples were evaluated for the concentrations of human Complement C5 in assay.
批内差异（Intra-Assay Statistics）
Ten replicates of each of three samples containing different Complement C5 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
批间差异（Inter-Assay Statistics）
Three samples containing different concentrations of Complement C5 were tested in independent assays. Acceptable criteria: CV<15%.
回收率（Recovery）
Recombinant Complement C5 was spiked into three diluted human serum samples, and then analyzed. The average recovery of Complement C5 for serum samples is 97.4%.
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