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产品参数（Product Specifications）
Assay TypeSandwich-ELISAAnalyteAAV2Format96TRegulatory StatusRUOSensitivity<3.13E+08 capsids/mLStandard Curve Range1.00E+10 capsids/mL-3.13E+08 capsids/mLAssay Time2 hr 20 minSuitable Sample TypeFor the detection and quantitative detection and quantitative determination of AAV2 capsid titration in AAV gene therapy product preparation processing.Sample volume100 μL产品概述（Product Overview）
AAV2 Titration ELISA Kit (Fast) is based on the sandwich-ELISA method and is used to detect and quantitatively determine the AAV2 capsid titration in AAV gene therapy product winthin 2h 20min. The kit is designed to provide reliable solutions for the early development, production, and quality control of AAV gene therapy products.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
组分（Materials Provided）
IDComponentsSizeAAV02H-C01Pre-coated Anti-AAV2 Antibody Microplate1 plateAAV02H-C02AAV2 Standard5.00E+10 capsidsAAV02H-C03HRP-Anti-AAV2 Antibody15 μgAAV02H-C0410×Washing Buffer50 mLAAV02H-C052×Dilution Buffer50 mLAAV02H-C06Substrate Solution12 mLAAV02H-C07Stop Solution7 mL质量管理控制体系（QMS）
数据展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
稀释线性（Dilution Linearity）
To assess the linearity of the assay, samples spiked with high concentrations of AAV2 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
批内差异（Intra-Assay Statistics）
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.
批间差异（Inter-Assay Statistics）
Three samples of known concentration were tested in three separate assays to assess inter-assay precision.
回收率（Recovery）
Three AAV2 with different concentrations were tested to calculate the recovery rate.
专属性（Specificity）
The specific binding of AAV-A002H to AAV1/2/3/5/6/8/9/dj serotypes was detected. The results showed that AAV-A002H only binds specifically to AAV2.
The specific binding of AAV-A002H to native/denatured AAV2 was detected. The results showed that AAV-A002H only binds specifically to native AAV2. (Denatured AAV2: 95℃ for 5min)
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