Human HVEM (Luc) HEK293 Reporter Cell

1. Genetically modified cell lines best reflect MOA (Mechanism of Action)
2. Higher activity and larger assay window for robust and reproducible cell-based bioassay
3. Comprehensive application data to support assay development and validation
4. Full tracible record, stringent quality control and validated cell passage stability
5. Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
6. Global commercial license assistance whenever regulatory filing is required

描述（Description）

The Human HVEM (Luc) HEK293 Reporter Cell was engineered to not only express NF-kB signaling response element, but also express the receptor full length human HVEM (Uniprot: Q92956-1). When co-cultured with target cells expressing human BTLA, the BTLA/HVEM interaction drives NF-kB-mediated luminescence. Blocking the BTLA/HVEM interaction by either anti-BTLA or anti-HVEM antibodies results in a decrease in luminescence.

应用说明（Application）

• Screen for anti-human BTLA antibody or anti-human HVEM antibody blocking BTLA/HVEM interaction.

生长特性（Growth Properties）

Adherent

筛选标记（Selection Marker）

Hygromycin B (20 μg/mL)

培养基（Complete Growth Medium）

DMEM medium + 10% FBS

冻存液（Freeze Medium）

Serum-free cell cryopreservation medium

装量（Quantity）

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

存储（Storage）

Frozen in liquid nitrogen.

支原体检测（Mycoplasma Testing）

Negative

无菌检测（Sterility Testing）

Negative

使用说明（Instructions for Use）

See data sheet for detailed culturing and assay protocol.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程

LIMITED USE AND LICENSE STATEMENT

HEK293 Cell Line Products

By using any HEK293 cell line product purchased from ACRO Biosystems or its affiliates (collectively, "ACROBiosystems"), including the original 293 cells and any modified derivatives thereof (collectively, the "Product"), you (the "Customer") agree to be bound by the following terms of limited use. If you are not willing to accept these terms and the Product remains unused, please contact our customer service at customerservice@acrobiosystems.com to arrange for return of the unused Product for a refund.

• 1. Scope of Use

  For the use of the Product worldwide (excluding Mainland China, Hong Kong, Macao and Taiwan regions), Customer may use the Product for (i) internal research; (ii) drug discovery and assay development; (iii) internal quality assurance testing for biologic drugs and vaccines; and (iv) lot releasing assay for biologic drugs and vaccines, whether conducted by a commercial or non-commercial entity. Unless a separate sublicense has been obtained, it is NOT permitted to use the Product in (i) product manufacturing; (ii) provision of service, information, or data to an unaffiliated third party for a fee; (iii) resale of the Product or its derivatives, whether or not such Product or derivatives are resold for use in research or (iv) other commercial use not expressly permitted above.

  
  

  For the use of the Product in Greater China region (including Mainland China, Hong Kong, Macao and Taiwan regions), Customer may use the Product for internal research use only. Unless a separate sublicense has been obtained, it is NOT permitted to use the Product for any Commercial Use. For the purpose of this paragraph, "Commercial Use" means any and all uses of this product and derivatives by a party for profit or other consideration and may include but is not limited to use in: (i) product manufacture; (ii) provision of service, information or data to a third party for a fee; (iii) resale of the product or its derivatives, whether or not such Product or derivatives are resold for use in research; (iv) quality assurance testing for biologic drugs and vaccines; (v) quality control assays; (vi) lot releasing assay of biologic drugs and vaccines.

  
  

  If you require a sublicense for any use beyond the limited use permitted herein, including but not limited to commercial use, CRO/service provider activities for a fee, or any product manufacturing activities, please contact the local sales team of ACROBiosystems for further details.

  
  

  The Product is not intended for any animal or human therapeutic purposes, nor for any direct human in vivo use. The Product shall not be used in or administered to humans.

• 2. Transfer, Distribution and Sublicensing Restrictions

  Unless expressly permitted under a separate written agreement with/via ACROBiosystems, you have NO right to (i) share, transfer, distribute, sell, resell, sublicense, or otherwise make the Product or its derivatives available for use to any third party, including but not limited to other researchers, laboratories, departments, research institutions, hospitals, universities, contract research organizations (CROs), contract development and manufacturing organizations (CDMOs), or any other commercial entities; or (ii) use the Product or its derivatives to perform fee-for-service activities or to provide services, information, or data to any third party for monetary or other consideration.

• 3. Modification and Derivatives

  Customers have No right to modify, engineer, genetically alter, or create derivatives of the Product. Any such modification or derivative requires a separate license and additional fees. Please contact our local sales team for further details.

• 4. No Warranty

  ACROBiosystems makes no warranties or representations of any kind, either expressed or implied, with respect to the merchantability, fitness or suitability of the Product for any particular purpose.

• 5. Limitation of Liability

  To the fullest extent permitted by law, in no event shall ACROBiosystems or its licensors be liable for any use by Customer of the Product, any derivative thereof, or any material treated therewith for any losses, costs, claims, damages, or liability of whatsoever kind or nature which may arise from or in connection with the use of the Product.

• 6. Compliance with Laws

  Customer agrees to use the Product in compliance with all applicable laws, regulations, and governmental guidelines applicable to the Product, any derivative thereof, and any materials treated therewith.

ACKNOWLEDGMENT

By using this Product, Customer acknowledges that he/she has read, understood, and agreed to be bound by the terms and conditions of this Limited Use and License Statement. If Customer does not agree to comply with these terms, Customer shall not open or use the Product and shall contact ACROBiosystems to arrange for return of the unused Product.