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- ProductSizeAmountActiveMax® Human MSLN μBeads, premium grade (for cells)2.5 mg2.5×10⁷ beadsActiveMax® Human MSLN μBeads, premium grade (for cells)10 mg (2.5 mg×4)1.0×10⁸ beads
产品描述（Product Description）
ActiveMax® Human MSLN μBeads, premium grade (for cells) are uniform, superparamagnetic beads of 5.5 µm in diameter immobilized with Human MSLN protein expressed from human 293 cells (HEK293) and contains AA Glu 296 - Gly 580 (Accession # AAH09272.1).
ActiveMax® Human MSLN μBeads, premium grade (for cells) are produced under sterile manufacturing conditions (ISO 5), and no animal- or human-derived components are used throughout the production process. It is produced under our rigorous quality control system that includes a comprehensive set of tests including sterility and endotoxin tests.应用说明（Application）
ActiveMax® Human MSLN μBeads, premium grade (for cells) are designed to stimulate in vitro MSLN-specific CAR-T cells or UCAR-T cells, similar to the tumor cell lines that express human MSLN antigen. It can be used as follows:
Evaluating the characteristics of CAR-T cells or UCAR-T cells.
In vitro expansion of MSLN-specific CAR-T cells or UCAR-T cells.
In vitro enrichment of MSLN-specific CAR-T cells or UCAR-T cells.重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
This product is stable in storage under the following conditions: -20˚C for 12 months in lyophilized state. -70°C for 3 months under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles after reconstitution. Immediate use after reconstitution is highly recommended.
无菌（Sterility）
Negative
内毒素（Endotoxin）
Less than 0.002 EU per μg by the LAL method / rFC method.
支原体（Mycoplasma）
Negative
注意事项（Important Note）
This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
质量管理控制体系（QMS）
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数据展示
流式数据 -FACS Data
Assay of human MSLN protein on the μBeads surface by Flow cytomtry. ActiveMax® Human MSLN μBeads, premium grade (for cells) (Cat. No. MBS-C003) were stained with anti-human MSLN antibody, and then followed by FITC anti-human IgG Fc antibody and analyzed by flow cytometry.
Protocol
刺激Mesothelin CAR-T细胞 -Cytokines
ActiveMax® Human MSLN μBeads, premium grade (for cells) (Cat. No. MBS-C003) can activate MSLN-specific CAR-T cells by detecting the secretion of IFN- γ in vitro.
MSLN CAR-T cells were stimulated with ActiveMax® Human MSLN μBeads (Cat. No. MBS-C003) for 24h, the cell-free supernatant was collected by centrifugation for evaluating IFN-γ, TNFα and IL-4 secretion by CBA. The results indicated that MSLN CAR-T cells responded to MSLN μBeads, and the expressions of the IFN-γ, TNFα and IL-4 increased (Data is the average of 3 independent experiments, error bars ± SD).
刺激Mesothelin CAR-T细胞 -Cytotoxic Molecules
MSLN CAR-T cells were stimulated with ActiveMax® Human MSLN μBeads (Cat. No. MBS-C003) for 24h, the cell-free supernatant was collected by centrifugation for evaluating Granzyme B secretion by ELISA. The results indicated that MSLN CAR-T cells responded to MSLN μBeads, and the expressions of the Granzyme B increased (Data is the average of 3 independent experiments, error bars ± SD).
刺激Mesothelin CAR-T细胞 -Activation Marker
MSLN CAR-T cells were stimulated with ActiveMax® Human MSLN μBeads (Cat. No. MBS-C003) for 24h. After demagnetization of the beads, the cells were collected and CD25, CD69, and CD137 were detected by FACS. The results indicated that MSLN CAR-T cells responded to MSLN μBeads, and the expressions of the three markers increased (Data is the average of 3 independent experiments, error bars ± SD).
刺激Mesothelin CAR-T细胞 -Exhaustion Marker
MSLN CAR-T cells were stimulated with ActiveMax® Human MSLN μBeads (Cat. No. MBS-C003) for 24h. After demagnetization of the beads, the cells were collected and LAG3, PD-1 were detected by FACS. The results indicated that MSLN CAR-T cells responded to MSLN μBeads, and the expressions of the two markers increased (Data is the average of 3 independent experiments, error bars ± SD).
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