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- ProductSizeAmountActiveMax® DLL4 μBeads, premium grade (for cells, 20 μm)2.5 mg7.5×10⁵ beadsActiveMax® DLL4 μBeads, premium grade (for cells, 20 μm)10 mg (2.5 mg×4)3.0×10⁶ beads
产品描述（Product Description）
ActiveMax® Human DLL4 μBeads, premium grade (for cells, 20 μm) are uniform, superparamagnetic beads of 20 µm in diameter immobilized with Human DLL4 Protein, expressed from human 293 cells (HEK293) and contains AA Ser 27 - Pro 524 (Accession # NP_061947.1 ).
ActiveMax® Human DLL4 μBeads, premium grade (for cells, 20μm) are produced under sterile manufacturing conditions (ISO 5) , and no animal- or human-derived components are used throughout the production process. It is produced under our rigorous quality control system that includes a comprehensive set of tests including sterility and endotoxin tests.应用说明（Application）
ActiveMax® Human DLL4 μBeads, premium grade (for cells, 20 μm) is designed to synergistically activate Notch signaling in hematopoietic stem/progenitor cells (HSPCs). The synergistic interactions between Notch ligand Delta-like 4 and VCAM-1 are leveraged to enhance Notch signaling to support efficient T-cell lineage commitment, so the beads is recommended to use for in vitro production of induced pluripotent stem cell derived T cells.
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
This product is stable in storage under the following conditions: -20˚C for 12 months in lyophilized state. -70°C for 3 months under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles after reconstitution. Immediate use after reconstitution is highly recommended.
无菌（Sterility）
Negative
内毒素（Endotoxin）
Less than 0.04 EU per μg by the LAL method / rFC method.
支原体（Mycoplasma）
Negative
注意事项（Important Note）
This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
质量管理控制体系（QMS）
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数据展示
流式数据 -FACS Data
Assay of human DLL4 protein on the μBeads surface by Flow cytometry. Add 100ul of 1:80 Anti-DLL4 Antibody/PE-anti-Mouse IgG1 dilution into 5e5 of ActiveMax® Human DLL4 μBeads, premium grade (for cells, 20 μm)(Cat. No. MBS-C024), negative control as well. PE signals was used to evaluate DLL4 beads activity (QC tested).
Protocol
应用数据（Application Data）
CD34+ CD45+ hematopoietic cells were co-cultured with Human DLL4 μBeads (for cells, 20 μm) (Cat. No. MBS-C024) and differentiated over a 14-day period. Subsequent flow cytometric analysis was performed to evaluate the expression of T-cell progenitor markers CD5 and CD7. The results indicated that Human DLL4 μBeads (for cells, 20 μm) (Cat. No. MBS-C024) , in combination with supplementary growth factors, effectively promoted the differentiation of CD7+ cells as well as CD5+CD7+ double-positive T-cell progenitors. The induction efficiency was enhanced as the cell-to-bead ratio increased from 40:1 to 1:3. Based on both induction performance and cost-effectiveness, a cell-to-bead ratio of approximately 1:3 is recommended.
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