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- ProductSizeAmountActiveMax® Human CD19 μBeads, premium grade (for cells, Super stable)2.5 mg2.5 × 10⁷ beadsActiveMax® Human CD19 μBeads, premium grade (for cells, Super stable)10 mg (2.5 mg × 4)1.0 × 10⁸ beads
产品展示（Product Show）
This product can be directly isolated using conventional column-free magnetic stand (eg. Cat. MB-01/ MB-02/ MB-03/ MB-04). Please note that due to its micron-scale size, it is not compatible with commercially available column-based (M/D/L) magnetic separation systems.
优势特色（Features）
- Sterility
- Endotoxin content ≤0.002 EU/μg
- Mycoplasma
- Batch-to-batch consistency
产品描述（Product Description）
ActiveMax® Human CD19 μBeads, premium grade (for cells) are uniform, superparamagnetic beads of 5.5 µm in diameter immobilized with Human CD19 protein expressed from human 293 cells (HEK293) and contains AA Pro 20 - Pro 278 (Accession # P15391-1).
ActiveMax® Human CD19 μBeads, premium grade (for cells) are produced under sterile manufacturing conditions (ISO 5), and no animal- or human-derived components are used throughout the production process. It is produced under our rigorous quality control system that includes a comprehensive set of tests including sterility and endotoxin tests.
Compared to MBS-C002, this product demonstrates superior stability and a longer shelf life, ensuring consistent and reliable performance over extended periods.应用说明（Application）
ActiveMax® Human CD19 μBeads, premium grade (for cells) are designed to stimulate in vitro CD19-specific CAR-T cells or UCAR-T cells, similar to the tumor cell lines that express human CD19 antigen. It can be used as follows:
Evaluating the characteristics of CAR-T cells or UCAR-T cells.
In vitro expansion of CD19-specific CAR-T cells or UCAR-T cells.
In vitro enrichment of CD19-specific CAR-T cells or UCAR-T cells.存储（Storage）
This product is stable in storage under the following conditions: -20˚C for 12 months in lyophilized state. -70°C for 12 months under sterile conditions after reconstitution. 2-8°C for 2 week under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles after reconstitution. Immediate use after reconstitution is highly recommended.
无菌（Sterility）
Negative
内毒素（Endotoxin）
Less than 2 EU per mg by the LAL method / rFC method.
支原体（Mycoplasma）
Negative
注意事项（Important Note）
This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
质量管理控制体系（QMS）
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数据展示
流式数据 -FACS Data
Assay of human CD19 protein on the μBeads surface by Flow cytomtry. The human CD19 conjugated on the μBeads (Cat. No. MBS-C035) surface were fluorescently stained using PE labeled anti-human CD19 antibody and analyzed by flow cytometry (QC tested).
Protocol
应用数据（Application Data）
ActiveMax® Human CD19 μBeads, premium grade (for cells, Super stable) (Cat. No. MBS-C035) can activate CD19-specific CAR-T cells by detecting the secretion of IFN- γ in vitro (Routinely tested).
Enrichment of Anti-CD19+ CAR-T Cells Using ActiveMax® Human CD19 μBeads, Premium Grade (for cells, Super stable).
For 2.5 × 10⁶ Anti-CD19 CAR-T cells with initial positivity rates of 59.05% (donor 1)and 31.18% (donor 2), respectively, significant enrichment was achieved using ActiveMax® Human CD19 μBeads, Premium Grade (for cells, Super stable) (Cat. No. MBS-C035) at Beads : cells ratios of 0.2:1, 1:1, and 5:1. At a Beads : cells ratio of 5:1, the enrichment efficiency reached 87% for the donor 1 and 95% for the donor 2.
Beads enrichment efficiency=1-(untouched cells * ratio of CD19 CAR-T )/(Initial cells * ratio of CD19 CAR-T) * 100%.
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