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- ProductSizeAmountCellectra™ Human CD14 NanoSort Microbeads, Research grade2 mL Reconstitution BufferFor 10⁹ total cells
产品展示（Product Show）
This product is compatible with mainstream automated cell processing instruments and commercially available column-based (M/D/L) magnetic separation systems.
产品描述（Product Description）
Cellectra™ Human CD14 NanoSort Microbeads are 50 nm dextran-coated superparamagnetic iron oxide nanoparticles which the surface is conjugated with recombinant monoclonal antibodies specific to human CD14 (Isotype: mouse IgG1). They are especially designed for positive selection of CD14+ cells.
Cellectra™ Human CD14 NanoSort Microbeads are produced under sterile manufacturing conditions (ISO 5), and no animal- or human-derived components are used throughout the production process. It is produced under our rigorous quality control system that includes a comprehensive set of tests including sterility and endotoxin tests.应用说明（Application）
Cellectra™ Human CD14 NanoSort Microbeads are designed for the positive selection of monocytes and macrophages from fresh or frozen human peripheral blood mononuclear cells (PBMCs). CD14, a glycosylphosphatidylinositol (GPI)-anchored membrane protein, is predominantly expressed on the surface of human monocytes and macrophages. The magnetic nBeads are conjugated with monoclonal antibodies targeting human CD14 protein, enabling CD14+ cells to be labeled with the specific antibodies and magnetic particles, and separated using the separation columns and magnets.
存储（Storage）
This product is stable after storage at: 2-8°C for 36 months in lyophilized state; 2-8°C for 18 months under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles once reconstituion.
无菌（Sterility）
Negative
内毒素（Endotoxin）
Less than 0.002 EU per μL by the LAL method / rFC method.
支原体（Mycoplasma）
Negative
注意事项（Important Note）
This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
制剂（Formulation）
Lyophilized in PBS with 0.1% recombinant HSA, pH 7.4. Trehalose is added as protectant before lyophilization.
质量管理控制体系（QMS）
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数据展示
流式数据 -FACS Data
The purity of CD14+ cells isolated by Cellectra™ Human CD14 NanoSort Microbeads, Research grade (Cat. No. MBS-S001).
The CD14+ cells were isolated from human PBMCs using Cellectra™ Human CD14 NanoSort Microbeads, Research grade (Cat. No. MBS-S001). The isolated cells and non-isolated cells were respectively stained using PE anti-human CD14 antibody, and then analyzed by FCS Express 7 software.Protocol
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