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抗体来源（Source）
Monoclonal Anti-Coxsackievirus A16 (strain G-10) VP0 Antibody, Human IgG1 (14E1) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
克隆号（Clone）
14E1
种属（Species）
Mouse
亚型（Isotype）
Human IgG1 | Human Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Virus
免疫原（Immunogen）
Recombinant Coxsackievirus A16 (strain G-10) VP0 Protein is expressed from human 293 cells.
特异性（Specificity）
Specifically recognizes Coxsackievirus A16 (strain G-10) VP0 Protein.
应用（Application）
ApplicationRecommended UsageWestern Blot10-0.02 μg/mLELISA0.1-63 ng/mL纯度（Purity）
95% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
交叉验证（Cross Verification）
The antibody can be used for WB detection.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Monoclonal Anti-Coxsackievirus A16 (strain G-10) VP0 Antibody, Human IgG1 (14E1) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-Coxsackievirus A16 (strain G-10) VP0 Antibody, Human IgG1 (14E1) (Cat. No. VP0-MY2108) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Coxsackievirus A16 (strain G-10) VP0 Protein, His Tag (Cat. No. VP0-C52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Coxsackievirus A16 (strain G-10) VP0 Antibody, Human IgG1 (14E1) (Cat. No. VP0-MY2108) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
Western Blot验证
Detection of Monoclonal Anti-Coxsackievirus A16 (strain G-10) VP0 Antibody, Human IgG1 (14E1) by Western Blot. Monoclonal Anti-Coxsackievirus A16 (strain G-10) VP0 Antibody, Human IgG1 (14E1) at 0.02 μg/mL dilution + Coxsackievirus A16 (strain G-10) VP0 Protein, His Tag at 400 ng.
Secondary: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.
Predicted band size: 40-60 kDa 12% Bis-Tris Protein Gel.
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