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Anti-SARS-CoV-2 Spike RBD Broadly Antibody, Mouse IgG1 (2G7F5) (MALS verified)

COADS MS
DS
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SPD-Y68-100ug
¥3675.00
SPD-Y68-1mg
¥24255.00
合计0件 产品金额¥ 0
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产品详情

  • 抗体来源（Source）

    Anti-SARS-CoV-2 Spike RBD Broadly Antibody, Mouse IgG1 Mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Spike protein.

  • 克隆号（Clone）

    2G7F5

  • 种属（Species）

    Mouse

  • 亚型（Isotype）

    Mouse IgG1 | Mouse Kappa

  • 偶联（Conjugate）

    Unconjugated

  • 抗体类型（Antibody Type）

    Hybridoma Monoclonal

  • 免疫原（Immunogen）

    Recombinant SARS-CoV-2 Spike Trimer Protein (BA.4/Omicron) erived from human 293 cells

  • 特异性（Specificity）

    This product can broadly reacts with SARS-CoV-2 Spike protein of WT and variant.

  • 应用（Application）

    Application
    Recommended Usage
    ELISA
    0.1-75 ng/mL

  • 纯度（Purity）

    95% as determined by SDS-PAGE.

    90% as determined by SEC-MALS.

  • 纯化（Purification）

    Protein A purified / Protein G purified

  • 制剂（Formulation）

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

    Contact us for customized product form or formulation.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳（SDS-PAGE）

    Spike RBD SDS-PAGE

    Anti-SARS-CoV-2 Spike RBD Broadly Antibody, Mouse IgG1 (2G7F5) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Spike RBD SEC-MALS

    The purity of Anti-SARS-CoV-2 Spike RBD Broadly Antibody, Mouse IgG1 (2G7F5) (Cat. No. SPD-Y68) is more than 90% and the molecular weight of this protein is around 126-154 kDa verified by SEC-MALS.

    Report

  • 活性（Bioactivity）-ELISA

    Spike RBD ELISA

    Immobilized SARS-CoV-2 (COVID-19) S protein RBD (Cat. No. SPD-C52H3), Alpha (Cat. No. SPD-C52Hn), Beta (Cat. No. SPD-C52Hp), Gamma (Cat. No. SPD-C52Hr), Delta (Cat. No. SPD-C52Hh), B.1.1.529/Omicron (Cat. No. SPD-C522e), BA.2/Omicron (Cat. No. SPD-C522g), BA.2.12.1/Omicron, BA.4&BA.5/Omicron (Cat. No. SPD-C522r), BA.3/Omicron and BA.2.75/Omicron (Cat. No. SPD-C522t) at 2μg/mL (100μL/well) can bind Anti-SARS-CoV-2 Spike RBD Broadly Antibody, Mouse IgG1 (2G7F5) (Cat. No. SPD-Y68) (QC tested).

    Protocol

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背景介绍

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

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