关注公众号
收藏产品 产品详情
抗体来源（Source）
Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1H09) is a rabbit monoclonal antibody recombinantly expressed in HEK293 cells.
抗体类型（Antibody Type）
Recombinant Monoclonal
克隆号（Clone）
M1H09
亚型（Isotype）
Rabbit IgG, Kappa
免疫原（Immunogen）
MMAE-BSA
特异性（Specificity）
Specifically recognizes MMAE and does not recognize MMAF.
纯化（Purification）
Protein A purified / Protein G purified.
浓度（Concentration）
Please refer to the Certificate of Analysis (CoA).
产品形式（Form）
Lyophilized
制剂（Formulation）
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
重构方法（Reconstitution）
Please refer to the Certificate of Analysis (CoA) for specific instructions.
运输（Shipping）
Lyophilized product is shipped at ambient temperature.
存储（Storage）
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
合规声明（Notices）
Product Specific Notices: For research use only.
质量管理控制体系（QMS）
推荐产品
数据展示
活性（Bioactivity）-ELISA
Immobilized Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1H09) (Cat. No. MME-MY2209) at 5 μg/mL, add Disitamab Vedotin (RC48) in the 100% Human Serum and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Protocol
Immobilized Disitamab Vedotin (RC48) at 0.2 μg/mL (100 μL/well) can bind Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1H09) (Cat. No. MME-MY2209) with a linear range of 0.06-1 ng/mL (Routinely tested).
Protocol
Immobilized Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1H09) (Cat. No. MME-MY2209) at 5 μg/mL, add Disitamab Vedotin (RC48) in the 0.5% BSA and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (Routinely tested).
Protocol
Serial dilutions of Monomethyl auristatin E were added into Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1H09) (Cat. No. MME-MY2209): Disitamab Vedotin (RC48) binding reactions. The half maximal inhibitory concentration (IC50) is 0.007529 μg/mL (Routinely tested).
Protocol
交叉验证-Cross Verification
ELISA binding of Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1H09) (Cat. No. MME-MY2209) with Disitamab Vedotin (RC48), Trastuzumab Deruxtecan, Sacituzumab Govitecam, Trastuzumab MMAE and Trastuzumab-DM1 conjugated antibody respectively.
The coating antibody was Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1H09) (Cat. No. MME-MY2209), used at 1 μg/mL concentration. The primary antibody were different payload conjugated antibodies, including Disitamab Vedotin (RC48), Trastuzumab Deruxtecan, Sacituzumab Govitecam, Trastuzumab MMAE and Trastuzumab-DM1 conjugated antibodies used at 0.5 μg/mL concentration. The secondary antibody was HRP conjugated Anti-Human-IgG-Fc Antibody (6F11C8), mAb (Acro, Cat. No. IGG-LY69) used at 1:10000 concentration.
Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1H09) (Cat. No. MME-MY2209) is specific to Disitamab Vedotin (RC48) and Trastuzumab MMAE, and has no cross-reactivity with Trastuzumab Deruxtecan, Sacituzumab Govitecam and Trastuzumab-DM1 (Routinely tested).
用户评价 发表评论
联系我们 
项目合作 