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抗体来源（Source）
Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
亚型（Isotype）
Mouse IgG1 | Mouse kappa
偶联（Conjugate）
Biotin
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Chemical
免疫原（Immunogen）
MMAE
特异性（Specificity）
Specifically recognizes the target-MMAE&MMAF.
应用（Application）
ApplicationRecommended UsageELISA2-250 ng/mL纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
活性（Bioactivity）-ELISA
Immobilized Disitamab Vedotin (RC48) at 1 μg/mL (100 μL/well) can bind Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-BLS104) with a linear range of 2-31 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-BLS104) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Disitamab Vedotin (RC48) with a linear range of 0.0078-0.125 ng/mL (Routinely tested).
Protocol
Immobilized Human Her2, His Tag (Cat. No. HE2-H5225) at 1 μg/mL, add Disitamab Vedotin (RC48) in the 100% Human Serum and then add Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-BLS104) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Protocol
Immobilized Human Her2, His Tag (Cat. No. HE2-H5225) at 1 μg/mL, add Disitamab Vedotin (RC48) in the 0.5% BSA and then add Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-BLS104) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (Routinely tested).
Protocol
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