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抗体来源（Source）
Monoclonal Anti-Human IgG3 Fc Antibody, Mouse IgG2a (2B7) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
克隆号（Clone）
2B7
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG2a | Mouse Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Human
免疫原（Immunogen）
Recombinant Human IgG3 Fc is expressed from human 293 cells
特异性（Specificity）
Specifically recognizes IgG3 Fc.
应用（Application）
ApplicationRecommended UsageWestern Blot10-0.1 μg/mLELISA0.1-125 ng/mL纯度（Purity）
90% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
交叉验证（Cross Verification）
This product No cross-reactivity in ELISA with
Anti-CD4 Antibody (Ibalizumab-IgG4).
Monoclonal Anti-IFNγ Antibody, Human IgG1 (8C5F8) (Cat. No. IFN-M412).
Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG2 (AM359b) (Cat. No. SPD-M400a).
Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG2 (AM359b) (Cat. No. SPD-M400a).
Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgA1 (AM130) (Cat. No. S1N-M164).
Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Cynomolgus IgG1 (AM122) (Cat. No. SPD-M201).
Monoclonal Anti-Eribulin Antibody, Rabbit IgG (1M1G11) (Cat. No. ERN-MY2012b).
Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgM (AM122) (Cat. No. SPD-M162).
Human CD3 epsilon, Mouse IgG2a Fc Tag (Cat. No. CDN-H5253).
Human Cadherin-17 Protein, Mouse IgG1 Fc Tag (Cat. No. CA7-H52A9).质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Monoclonal Anti-Human IgG3 Fc Antibody, Mouse IgG2a (2B7) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-Human IgG3 Fc Antibody, Mouse IgG2a (2B7) (Cat. No. IG3-MY2179) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG3 (AS35) (Cat. No. SPD-S67) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human IgG3 Fc Antibody, Mouse IgG2a (2B7) (Cat. No. IG3-MY2179) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Western Blot验证
Detection of Monoclonal Anti-Human IgG3 Fc Antibody, Mouse IgG2a (2B7) by Western Blot. Monoclonal Anti-Human IgG3 Fc Antibody, Mouse IgG2a (2B7) at 0.1 μg/mL dilution + Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG3 (AM359b) at 400 ng.
Secondary: (MFC)-Peroxidase AffiniPure GoatAnti-MouseIgG,FcγFragmentSpecificat 1/1500 dilution
Predicted band size: 60 kDa 12% Bis-Tris Protein Gel
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