Monoclonal Anti-Mouse IgG2b Antibody, Rabbit IgG (P1F04) (MALS verified)

用户评价
货号-规格
价格
Qty.
IGB-MY2176-100ug
¥2200.00
IGB-MY2176-1mg (500ug X 2)
¥16000.00
合计0件 产品金额¥ 0

产品详情

  • 抗体来源(Source)

    Monoclonal Anti-Mouse IgG2b Antibody, Rabbit IgG (P1F04) is a Rabbit monoclonal antibody recombinantly expressed from HEK293 cells.

  • 克隆号(Clone)

    P1F04

  • 种属(Species)

    Rabbit

  • 亚型(Isotype)

    Rabbit IgG | Rabbit Kappa

  • 偶联(Conjugate)

    Unconjugated

  • 抗体类型(Antibody Type)

    Recombinant Monoclonal

  • 种属反应性(Reactivity)

    Specifically recognizes Mouse IgG2b Fc,no recrecognizes Mouse IgG1 Fc,Mouse IgG2a Fc and Mouse IgG3 Fc

  • 免疫原(Immunogen)

    Recombinant Mouse IgG2b Fc Protein is expressed from human 293T cells

  • 特异性(Specificity)

    This product is a specific antibody specifically reacts with IgG2b.

  • 应用(Application)

    Application
    Recommended Usage
    Western Blot
    0.02-10 μg/mL
    ELISA
    0.1-63 ng/mL
  • 纯度(Purity)

    95% as determined by SDS-PAGE.

    90% as determined by SEC-MALS.

  • 纯化(Purification)

    Protein A purified / Protein G purified

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 交叉验证(Cross Verification)

    This product No cross-reactivity in ELISA with

    Mouse IgG Fc, Tag Free (Cat. No. IG1-M5208).

    Mouse IgG2a Fc, Tag Free (Cat. No. IGA-M5207).

    Mouse IgG3 Fc Protein, Tag Free (Cat. No. IG3-M5214).

    Mouse IgE Fc Protein, His Tag (Cat. No. IGE-M52H3).

    Monoclonal Anti-Human p-tau217 Antibody, Mouse IgG1 (6G7C8) (Cat. No. TAU-MY2102).

    Human IgG Fc, Tag Free (Cat. No. FCC-H5214).

    Human IgG2 Fc, Tag Free (Cat. No. IG2-H5206).

    Human IgG3 Fc, Tag Free (Cat. No. IG3-H5200).

    Human IgG4 Fc, Tag Free (Cat. No. IG4-H5205).

    Mouse IgG3 Fc, Tag Free (Cat. No. IG3-M5201).

    Mouse IgG3 Fc Protein, Tag Free (Cat. No. IG3-M5214).

  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

推荐产品

数据展示

  • 电泳(SDS-PAGE)

    IgG2b SDS-PAGE

    Monoclonal Anti-Mouse IgG2b Antibody, Rabbit IgG (P1F04) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IgG2b SEC-MALS

    The purity of Monoclonal Anti-Mouse IgG2b Antibody, Rabbit IgG (P1F04) (Cat. No. IGB-MY2176) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     IgG2b ELISA

    Immobilized Mouse IgG2b Fc, Tag Free (Cat. No. IGB-M5203) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Mouse IgG2b Antibody, Rabbit IgG (P1F04) (Cat. No. IGB-MY2176) with a linear range of 0.1-2 ng/mL (QC tested).

    Protocol
  • Western Blot验证

     IgG2b WESTERN BLOT
  • Detection of Monoclonal Anti-Mouse IgG2b Antibody, Rabbit IgG (P1F04) (MALS verified)by Western Blot. Monoclonal Anti-Mouse IgG2b Antibody, Rabbit IgG (P1F04) (MALS verified) at 0.02 μg/mL dilution + Mouse IgG2b Fc Protein, Tag Free (MALS verified) at 400 ng.

    Secondary: (GAR)-HRP Goat Anti-Rabbit IgG, Fc fragment specific (min X Hu Sr Prot) 111-035-046 1/2000 dilution

    Predicted band size: 33kDa-38kDa 12% Bis-Tris Protein Gel

用户评价
发表评论

背景介绍

Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b.

前沿进展

 
联系我们
项目合作
查看更多项目
实验案例分析
查看更多案例分析
  • 产品基础信息
  • 产品详情
  • 推荐产品
  • 数据展示
  • 用户评价
  • 背景介绍