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抗体来源（Source）
Monoclonal Rabbit IgG Antibody Isotype Control is a mouse monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply.
应用（Application）
Flow Cytometry.
种属（Species）
Rabbit
亚型（Isotype）
Rabbit IgG | Rabbit Kappa
特异性（Specificity）
Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins, which is normally not present in Humans or animals. Therefore, DNP-AFM776 does not react with any antigen of Human or animal cells.
免疫原（Immunogen）
DNP (Dinitrophenyl)
偶联（Conjugate）
AF647
Excitation Wavelength: 640 nm
Emission Wavelength: 672 nm
推荐稀释比（Recommended Dilution）
1:50
制剂（Formulation）
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300, with trehalose as protectant.
Please contact us for customized product forms or formulations.
重构方法（Reconstitution）
Please refer to the Certificate of Analysis (CoA) for specific instructions.
For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution.
- 2-8 °C for 12 months after reconstitution.
质量管理控制体系（QMS）
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数据展示
活性（Bioactivity）-FACS
Flow cytometric analysis of Anti-CD19 CAR-293 Cells staining with Alexa Fluor 647-Labeled Rabbit IgG Antibody Isotype Control (Cat. No. DNP-AFM776) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with positive control antibody. Alexa Fluor 647 signal was used to evaluate the binding activity (QC tested).
Protocol
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