Human IgG2 (A330S, P331S) Kappa Isotype Control (mAb, carrier free, MALS verified)

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Qty.
DNP-M884-100ug
¥1680.00
DNP-M884-1mg (500ug X 2)
¥7350.00
合计0件 产品金额¥ 0

产品详情

  • 抗体来源(Source)

    Human IgG2 (A330S, P331S) Kappa Isotype Control (mAb) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.

  • 种属(Species)

    Human

  • 亚型(Isotype)

    Human IgG2(A330S,P331S) | Human Kappa

  • 偶联(Conjugate)

    Unconjugated

  • 抗体类型(Antibody Type)

    Recombinant Monoclonal

  • 特异性(Specificity)

    This product is a specific antibody specifically reacts with DNP.

  • 应用(Application)

    Application
    Recommended Usage
    ELISA
    0.05-13 ng/mL
  • 纯度(Purity)

    95% as determined by SDS-PAGE.

    90% as determined by SEC-MALS.

  • 纯化(Purification)

    Protein A purified / Protein G purified

  • 制剂(Formulation)

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • 重构方法(Reconstitution)

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

    For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    IgG control SDS-PAGE

    Human IgG2 (A330S, P331S) Kappa Isotype Control (mAb) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IgG control SEC-MALS

    The purity of Human IgG2 (A330S, P331S) Kappa Isotype Control (mAb) (Cat. No. DNP-M884) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     IgG control ELISA

    Immobilized Dinitrophenyl-BSA Conjugate (DNP-BSA) at 10 μg/mL (100 μL/well) can bind Human IgG2 (A330S, P331S) Kappa Isotype Control (mAb) (Cat. No. DNP-M884) with a linear range of 0.05-2 ng/mL (QC tested).

    Protocol

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背景介绍

Fc engineered isotype controls are designed to evaluate effector functions such as FcγR binding, FcRn interaction, ADCC, and CDC. Each variant is functionally validated, providing a reliable negative control framework for antibody mechanism studies and therapeutic development.

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