Human IgG1 (L234A, L235A) Kappa Isotype Control (mAb, carrier free, MALS verified)

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DNP-C18P1-100ug
¥1680.00
DNP-C18P1-1mg (250ug X 4)
¥7350.00
DNP-C18P1-25mg
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DNP-C18P1-100mg
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产品详情

  • 抗体来源(Source)

    Human IgG1 (L234A, L235A) Kappa Isotype Control (mAb) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.

  • 种属(Species)

    Human

  • 亚型(Isotype)

    Human IgG1(L234A,L235A) | Human Kappa

  • 偶联(Conjugate)

    Unconjugated

  • 抗体类型(Antibody Type)

    Recombinant Monoclonal

  • 特异性(Specificity)

    This product is a specific antibody specifically reacts with DNP.

  • 应用(Application)

    Application
    Recommended Usage
    ELISA
    0.1-60 ng/mL
  • 纯度(Purity)

    90% as determined by SDS-PAGE.

    95% as determined by SEC-MALS.

  • 纯化(Purification)

    Protein A purified / Protein G purified

  • 制剂(Formulation)

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

    25 mg or larger size will be supplied as liquid and shipped by dry ice. Please inquire the dry ice shipping cost.

  • 重构方法(Reconstitution)

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

    For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    IgG control SDS-PAGE

    Human IgG1 (L234A, L235A) Kappa Isotype Control (mAb) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IgG control SEC-MALS

    The purity of Human IgG1 (L234A, L235A) Kappa Isotype Control (mAb) (Cat. No. DNP-C18P1) is more than 95% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     IgG control ELISA

    Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Human IgG1 (L234A, L235A) Kappa Isotype Control (mAb) (Cat. No. DNP-C18P1) with a linear range of 0.1-4 ng/mL (QC tested).

    Protocol

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背景介绍

Fc engineered isotype controls are designed to evaluate effector functions such as FcγR binding, FcRn interaction, ADCC, and CDC. Each variant is functionally validated, providing a reliable negative control framework for antibody mechanism studies and therapeutic development.

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