Human IgG1 (M252Y, S254T, T256E) Kappa Isotype Control (mAb, carrier free, MALS verified)

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DNP-C12P1-100ug
¥1680.00
DNP-C12P1-1mg (500ug X 2)
¥7350.00
DNP-C12P1-25mg
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DNP-C12P1-100mg
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产品详情

  • 抗体来源(Source)

    Human IgG1 (M252Y, S254T, T256E) Kappa Isotype Control (mAb) is a chimeric monoclonal antibody recombinantly expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.

  • 抗体类型(Antibody Type)

    Recombinant Monoclonal

  • 亚型(Isotype)

    Human IgG1 (M252Y, S254T, T256E), Kappa

  • 宿主(Host Species)

    Human

  • 特异性(Specificity)

    This product is a specific antibody specifically reacts with DNP.

  • 纯化(Purification)

    Protein A purified / Protein G purified.

  • 聚合度(Aggregation)

    Less than 10%, as determined by SEC-MALS.

  • 浓度(Concentration)

    Please refer to the Certificate of Analysis (CoA).

  • 产品形式(Form)

    Lyophilized

  • 制剂(Formulation)

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

    25 mg or larger size will be supplied as liquid and shipped by dry ice. Please inquire the dry ice shipping cost.

  • 重构方法(Reconstitution)

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • 运输(Shipping)

    Lyophilized product is shipped at ambient temperature.

  • 存储(Storage)

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 合规声明(Notices)

    Product Specific Notices: For research use only.

  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 活性(Bioactivity)-ELISA

     IgG control ELISA

    Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Human IgG1 (M252Y, S254T, T256E) Kappa Isotype Control (mAb) (Cat. No. DNP-C12P1) with a linear range of 0.2-2 ng/mL (QC tested).

    Protocol
  • 活性(Bioactivity)-SPR

     IgG control SPR

    Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-H5286) captured on CM5 chip via anti-His antibody can bind Human IgG1 (M252Y, S254T, T256E) Kappa Isotype Control (mAb) (Cat. No. DNP-C12P1) with an affinity constant of 26.6 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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背景介绍

Fc engineered isotype controls are designed to evaluate effector functions such as FcγR binding, FcRn interaction, ADCC, and CDC. Each variant is functionally validated, providing a reliable negative control framework for antibody mechanism studies and therapeutic development.

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