APC-Labeled Monoclonal Anti-HLA class I Antibody, Mouse IgG2a (W632) (0.03% Proclin)

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价格
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HLI-AHFM816-25tests
¥480.00
HLI-AHFM816-200tests
¥2640.00
合计0件 产品金额¥ 0

产品详情

  • 抗体来源(Source)

    Monoclonal Anti-HLA class I Antibody, Mouse IgG2a (W632) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells, which provides higher batch consistency and long term security of supply.

  • 应用(Application)

    Flow Cytometry (Evaluation of the expression of HLA class I on Human cells).

  • 克隆号(Clone)

    W632

  • 种属(Species)

    Mouse

  • 亚型(Isotype)

    Mouse IgG2a | Mouse Kappa

  • 特异性(Specificity)

    This product is a specific antibody specifically reacts with HLA class I protein.

  • 种属反应性(Reactivity)

    Human

  • 免疫原(Immunogen)

    Purified HLA class I Protein

  • 偶联(Conjugate)

    APC

    Excitation Wavelength: 640 nm

    Emission Wavelength: 661 nm

  • 同型对照(Isotype Control)

    The Isotype control is sold separately and you can search for Cat. No. DNP-AFM487 for product information.

  • 推荐稀释比(Recommended Dilution)

    1:50

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 0.03% Proclin300, 0.2% BSA with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months after reconstitution.
    3. 2-8 °C for 12 months after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 活性(Bioactivity)-FACS

     HLA class I FACS

    Flow cytometric analysis of Human peripheral blood lymphocytes staining with APC-Labeled Monoclonal Anti-HLA class I Antibody, Mouse IgG2a (W632) (Cat. No. HLI-AHFM816) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 PBMCs in a final volume of 100 µL), compared with Isotype control antibody. APC signal was used to evaluate the binding activity (QC tested).

    Protocol

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背景介绍

The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains. Crossreactivity is also seen in baboon, rhesus and cynomolgus monkey.

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