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HRP conjugated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016)

COADS MS
DS
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G4S-HLY25G1-25ug
¥3675.00
G4S-HLY25G1-200ug
¥13840.00
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产品详情

  • 抗体来源（Source）

    HRP conjugated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) is a Rabbit monoclonal antibody recombinantly expressed from HEK293 cells.

  • 种属（Species）

    Rabbit

  • 亚型（Isotype）

    Rabbit IgG | Rabbit Kappa

  • 偶联（Conjugate）

    HRP-Conjugated

  • 抗体类型（Antibody Type）

    Recombinant Monoclonal

  • 应用（Application）

    Application
    Recommended Usage
    ELISA
    0.1-125 ng/mL

  • 纯度（Purity）

    95% as determined by SDS-PAGE.

  • 纯化（Purification）

    Protein A purified / Protein G purified

  • 制剂（Formulation）

    Please contact us for detailed information.

    Contact us for customized product form or formulation.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

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数据展示

  • 活性（Bioactivity）-ELISA

    G4S linker ELISA

    Immobilized Human LIGHT, His Tag (Cat. No. LIT-H5247) at 1 μg/mL (100 μL/well) can bind HRP conjugated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) (Cat. No. G4S-HLY25G1) with a linear range of 0.1-8 ng/mL (QC tested).

    Protocol

  • G4S linker ELISA

    Immobilized Human IL-33 Protein, His Tag (Cat. No. IL3-H52H7) at 1 μg/mL (100 μL/well) can bind HRP conjugated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) (Cat. No. G4S-HLY25G1) with a linear range of 0.1-8 ng/mL (Routinely tested).

    Protocol

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背景介绍

The G4S linker is emerged as the most common linking peptides for scFvs. It is composed of the repeated sequence (Gly-Gly-Gly-Gly-Ser) and commonly found as either a 15-mer (G4S)3 or 20-mer(G4S)4 within scFv fragments. The G4S linker has become an integral part of the vast majority of CARs as it links the VH and VL recognition domains of scFvs. The G4S linker is commonly used in CARs targeting solid tumor antigens, such as HER2 and CEA, while the whitlow/218 linker peptide is predominantly used in CAR constructs targeting hematological malignancies such as CD19 CAR products.

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