关注公众号
收藏产品 产品详情
抗体来源（Source）
Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) is a rabbit monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply. It shows superior performance in flow cytometry assays with higher affinity and better specificity.
应用（Application）
Flow Cytometry (Evaluation of cell surface expressed CARs of varying specificity containing a G4S linker within the scFv of the extracellular domain).
克隆号（Clone）
016
种属（Species）
Rabbit
亚型（Isotype）
Rabbit IgG | Rabbit Kappa
特异性（Specificity）
It can specifically recognize proteins or ScFv containing (G3S)n or (G4S)n linkers, where n = 2, 3, 4... That is, it can recognize linkers with a minimum length of 2 repeats for both (G3S)n and (G4S)n.
偶联（Conjugate）
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
推荐稀释比（Recommended Dilution）
1:50
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 0.03% Proclin300 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution.
- 2-8 °C for 12 months after reconstitution.
质量管理控制体系（QMS）
推荐产品
数据展示
活性（Bioactivity）-FACS
Flow cytometric analysis of Anti-MSLN CAR-293 cells staining with Alexa Fluor 488-Labeled Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) (Cat. No. G4S-AFYP1) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with Isotype control antibody. Alexa Fluor 488 signal was used to evaluate the binding activity (QC tested).
Protocol
Non-specificity of Alexa Fluor 488-Labeled Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) (Cat. No. G4S-AFYP1) binding to CD3+ cells present in human PBMC. 5e5 of human PBMCs were simultaneously stained with PE anti-CD3 antibody and Alexa Fluor 488-Labeled Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both PE and Alexa Fluor 488 positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).
Protocol
用户评价 发表评论
联系我们 
项目合作 