Monoclonal Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F)

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RSF-M309a-100ug
¥3675.00
RSF-M309a-1mg
¥24255.00
合计0件 产品金额¥ 0

产品详情

  • 抗体来源(Source)

    Monoclonal Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.

  • 克隆号(Clone)

    101F

  • 种属(Species)

    Mouse

  • 亚型(Isotype)

    Mouse IgG2a | Mouse Kappa

  • 偶联(Conjugate)

    Unconjugated

  • 抗体类型(Antibody Type)

    Recombinant Monoclonal

  • 特异性(Specificity)

    This product is a specific antibody specifically reacts with RSV-F0.

  • 应用(Application)

    Application
    Recommended Usage
    ELISA
    0.2-78 ng/mL
  • 纯度(Purity)

    95% as determined by SDS-PAGE.

  • 纯化(Purification)

    Protein A purified / Protein G purified

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 交叉验证(Cross Verification)

    This product can cross in Western Blot with

    HRSV (A) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H6).

    HRSV (A) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7).

    HRSV (B) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H9).

    HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8).

  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    Fusion glycoprotein F0/F protein (RSV) SDS-PAGE

    Monoclonal Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • 活性(Bioactivity)-ELISA

     Fusion glycoprotein F0/F protein (RSV) ELISA

    Immobilized HRSV (A) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H6) at 2 μg/mL (100 μL/well) can bind Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F) (Cat. No. RSF-M309a) with a linear range of 0.2-10 ng/mL (QC tested).

    Protocol
  •  Fusion glycoprotein F0/F protein (RSV) ELISA

    Immobilized HRSV (A) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) at 2 μg/mL (100 μL/well) can bind Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F) (Cat. No. RSF-M309a) with a linear range of 0.2-10 ng/mL (QC tested).

    Protocol

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背景介绍

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. The RSV fusion glycoprotein (RSV F) is the principal target of RSV neutralizing antibodies in human sera. The RSV F is a type I viral fusion protein synthesized as inactive, single-chain polypeptides that assemble into trimers. RSV F fuses the viral and host cell membranes by irreversible protein refolding from the labile prefusion conformation to the stable post-fusion conformation. Antibody 101F, target epitopes that have been mapped to linear regions in the F1 subunit, referred to as antigenic site IV.

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