Monoclonal Anti-HRSV F Antibody, Human IgG1 (4D7) (MALS verified)

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HRF-S268-100ug
¥3675.00
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产品详情

  • 抗体来源(Source)

    Monoclonal Anti-HRSV F Antibody, Human IgG1 (4D7) is a chimeric monoclonal antibody recombinantly expressed from CHO, which combines the variable region of a mouse monoclonal antibody with Human constant domain.

  • 克隆号(Clone)

    4D7

  • 种属(Species)

    Mouse

  • 亚型(Isotype)

    Human IgG1 | Human Kappa

  • 偶联(Conjugate)

    Unconjugated

  • 抗体类型(Antibody Type)

    Recombinant Monoclonal

  • 种属反应性(Reactivity)

    Virus

  • 特异性(Specificity)

    This product is a specific antibody specifically reacts with HRSV F.

  • 应用(Application)

    Application
    Recommended Usage
    ELISA
    0.2-125 ng/mL
  • 纯度(Purity)

    90% as determined by SDS-PAGE.

    95% as determined by SEC-MALS.

  • 纯化(Purification)

    Protein A purified / Protein G purified

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    Fusion glycoprotein F0/F protein (RSV) SDS-PAGE

    Monoclonal Anti-HRSV F Antibody, Human IgG1 (4D7) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Fusion glycoprotein F0/F protein (RSV) SEC-MALS

    The purity of Monoclonal Anti-HRSV F Antibody, Human IgG1 (4D7) (Cat. No. HRF-S268) is more than 95% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     Fusion glycoprotein F0/F protein (RSV) ELISA

    Immobilized Monoclonal Anti-HRSV F Antibody, Human IgG1 (4D7) (Cat. No. HRF-S268) at 1 μg/mL (100 μL/well) can bind HRSV (A) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H6) with a linear range of 0.2-8 ng/mL (QC tested).

    Protocol
  •  Fusion glycoprotein F0/F protein (RSV) ELISA

    Immobilized HRSV (B) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H9) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HRSV F Antibody, Human IgG1 (4D7) (Cat. No. HRF-S268) with a linear range of 0.05-1 ng/mL (Routinely tested).

    Protocol

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背景介绍

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. The RSV fusion glycoprotein (RSV F) is the principal target of RSV neutralizing antibodies in human sera. The RSV F is a type I viral fusion protein synthesized as inactive, single-chain polypeptides that assemble into trimers. RSV F fuses the viral and host cell membranes by irreversible protein refolding from the labile prefusion conformation to the stable post-fusion conformation. Antibody 4D7, which was found to bind antigenic site I on the postfusion form of RSV F.

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