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抗体来源（Source）
Rabbit Anti-Mouse FMC63 Polyclonal Antibody is a polyclonal antibody purified from rabbit serum.
种属（Species）
Rabbit
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Polyclonal Antibody
种属反应性（Reactivity）
Mouse
免疫原（Immunogen）
FMC63 scFv
特异性（Specificity）
Recognizes FMC63 scFv specifically.
应用（Application）
ApplicationRecommended UsageELISA0.1-10 ng/mL纯度（Purity）
90% as determined by SDS-PAGE.
95% as determined by SEC-MALS.
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Rabbit Anti-Mouse FMC63 Polyclonal Antibody on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Rabbit Anti-Mouse FMC63 Polyclonal Antibody (Cat. No. FM3-S93) is more than 95% and the molecular weight of this protein is around 130-145 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Mouse FMC63 Polyclonal Antibody (Cat. No. FM3-S93) with a linear range of 0.098-0.78 ng/mL (QC tested).
Protocol
Immobilized anti-CD19 antibody (Clone: FMC63) at 1 μg/mL, add increasing concentrations of Rabbit Anti-Mouse FMC63 Polyclonal Antibody (Cat. No. FM3-S93) and then add Biotinylated anti-CD19 antibody (Clone: FMC63) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 78 ng/mL (QC tested).
Protocol
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