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抗体来源（Source）
Monoclonal Anti-Mouse FMC63 antibody, Rabbit IgG1 (M4H08) is a rabbit monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply.
应用（Application）
Flow Cytometry.
克隆号（Clone）
M4H08
种属（Species）
Rabbit
亚型（Isotype）
Rabbit IgG | Rabbit Kappa
特异性（Specificity）
Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.
种属反应性（Reactivity）
Human
免疫原（Immunogen）
Recombinant FMC63 scFv derived from HEK293 cells
偶联（Conjugate）
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
同型对照（Isotype Control）
The Isotype control is sold separately and you can search for Cat. No. DNP-PFM776 for product information.
推荐稀释比（Recommended Dilution）
1:50
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 0.03% Proclin300, 0.2% BSA with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution.
- 2-8 °C for 12 months after reconstitution.
质量管理控制体系（QMS）
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数据展示
活性（Bioactivity）-FACS
Flow cytometric analysis of Anti-CD19 CAR-293 cells staining with PE-Labeled Monoclonal Anti-FMC63 Antibody, Rabbit IgG (M4H08) (Cat. No. FM3-PCFMY2) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared withisotype control antibody. PE signal was used to evaluate the binding activity (QC tested).
Protocol
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