Monoclonal Anti-Duocarmycin Antibody, Rabbit IgG (M1E06)

  • Antibody Internalization Detection Reagent (IGG-PZF2001) monitors endocytosis in live cells
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Qty.
DUN-MY2287-100ug
¥3150.00
DUN-MY2287-1mg (500ug X 2)
¥18900.00
合计0件 产品金额¥ 0

产品详情

  • 特异性(Specificity)

    Specifically recognizes the target-Duocarmycin.

  • 抗体来源(Source)

    Monoclonal Anti-Duocarmycin Antibody, Rabbit IgG (M1E06) is a Rabbit monoclonal antibody recombinantly expressed from HEK293 cells.

  • 克隆号(Clone)

    M1E06

  • 亚型(Isotype)

    Rabbit IgG | Rabbit Kappa

  • 偶联(Conjugate)

    Unconjugated

  • 免疫原(Immunogen)

    Duocarmycin-BSA

  • 纯化(Purification)

    Protein A purified / Protein G purified

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 活性(Bioactivity)-ELISA

     Duocarmycin ELISA

    Serial dilutions of Duocarmycin were added into BSA-Duocarmycin: Monoclonal Anti-Duocarmycin Antibody, Rabbit IgG (M1E06) (Cat. No. DUN-MY2287) binding reactions. The half maximal inhibitory concentration (IC50) is 0.10 μg/mL (QC tested).

    Protocol

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背景介绍

Duocarmycin, a DNA minor groove-binding alkylating agent, acts as a payload in antibody-drug conjugates (ADCs) by selectively targeting AT-rich regions of DNA. It forms covalent bonds with the N3 position of adenine, inducing strand breaks and apoptosis through S-phase cell cycle arrest . This mechanism bypasses multidrug resistance pathways, making it potent against aggressive tumors.

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