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HRP conjugated Monoclonal Anti-DM-1&DM-4 Antibody,Mouse IgG1

  • Antibody Internalization Detection Reagent (IGG-PZF2001) monitors endocytosis in live cells
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Qty.

DM1-PLY73-25ug
¥3675.00

DM1-PLY73-200ug
¥14490.00
合计0件 产品金额¥ 0

产品详情

  • 特异性(Specificity)

    This product is a specific antibody specifically reacts with DM-1 and DM-4.

  • 抗体来源(Source)

    HRP conjugated Monoclonal Anti-DM-1&DM-4 Antibody,Mouse IgG1 is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.

  • 亚型(Isotype)

    Mouse IgG1 | Mouse Kappa

  • 偶联(Conjugate)

    HRP-Conjugated

  • 种属反应性(Reactivity)

    Chemical

  • 免疫原(Immunogen)

    DM-1

  • 应用(Application)

    Application
    Recommended Usage
    ELISA
    1-500 ng/mL
  • 纯化(Purification)

    Protein A purified / Protein G purified

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 活性(Bioactivity)-ELISA

     DM-1 ELISA

    Immobilized Trastuzumab-DM1 (T-DM1) at 2 μg/mL (100 μL/well) can bind HRP conjugated Monoclonal Anti-DM-1&DM-4 Antibody,Mouse IgG1 (Cat. No. DM1-PLY73) with a linear range of 0.24-15.63 ng/mL (QC tested).

    Protocol
  •  DM-1 ELISA

    Immobilized Human Her2, His Tag (Cat. No. HE2-H5225) at 1 μg/mL, add Trastuzumab-DM1 in the 100% Human Serum and then add HRP conjugated Monoclonal Anti-DM-1&DM-4 Antibody,Mouse IgG1 (Cat. No. DM1-PLY73) at 0.5 μg/mL (QC tested).

    Protocol
  •  DM-1 ELISA

    Immobilized Human Her2, His Tag (Cat. No. HE2-H5225) at 1 μg/mL, add Trastuzumab-DM1 in the 0.5% BSA and then add HRP conjugated Monoclonal Anti-DM-1&DM-4 Antibody,Mouse IgG1 (Cat. No. DM1-PLY73) at 0.5 μg/mL (Routinely tested).

    Protocol

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背景介绍

Mertansine (DM-1) is a tubulin inhibitor that binds to the ends of microtubules and inhibits microtubule dynamics. DM-1(Mertansine) has antitumor activity and functions as a regulator of tubulin. It is an alpha-amino acid ester, a carbamate, an epoxide, an organic heterocyclic tetracyclic compound, an organochlorine compound, a mercaptan, and a maydenin alkaloid. DM-1, derived from Mydenin, is a cytotoxic component of antibody-drug conjugations that produce antibody-drug conjugations via a sulfhydryl group splice with SPP (n-succinimide 4- (2-pyridyl dithio)) or SMCC (4- (3-mercapto-2, 5-dioxy-1 pyrrolidyl) -cyclohexanic acid) splice.

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