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抗体来源（Source）
Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (UCHT1) is a monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply.
应用（Application）
ELISA (Evaluation of levels of CD3 protein).
Flow Cytometry (Detection the expression of CD3 on Human cells).
克隆号（Clone）
UCHT1
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1 | Mouse Kappa
特异性（Specificity）
This product is a specific antibody specifically reacts with CD3.
种属反应性（Reactivity）
Human
免疫原（Immunogen）
Purified Human CD3 Protein
偶联（Conjugate）
Unconjugated
同型对照（Isotype Control）
The Isotype control is sold separately and you can search for Cat. No. DNP-M1A1 for product information.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
活性（Bioactivity）-ELISA
Immobilized Human CD3E&CD3D Heterodimer Protein (Cat. No. CDD-H52Wa) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (UCHT1) (Cat. No. CDE-M532) with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
活性（Bioactivity）-FACS
2e5 of Jurkat cells were stained with 100 μL of 1 μg/mL of Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (UCHT1) (Cat. No. CDE-M532) and isotype control antibody respectively, washed and then followed by PE-anti mouse IgG1 antibody and analyzed with FACS (Routinely tested).
Protocol
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