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抗体来源（Source）
Anti c-Myc Tag Monoclonal Antibody (9E10) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
克隆号（Clone）
9E10
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Tag
特异性（Specificity）
This product is a specific antibody specifically reacts with c-Myc tag protein.
应用（Application）
ApplicationRecommended UsageWestern Blot10-0.02 μg/mLELISA0.2-250 ng/mL纯度（Purity）
95% as determined by SDS-PAGE.
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Anti c-Myc Tag Monoclonal Antibody (9E10) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human Serum Albumin C-10×His & C-Myc at 1 μg/mL (100 μL/well) can bind Anti c-Myc Tag Monoclonal Antibody (9E10) (Cat. No. MYC-MMYCc) with a linear range of 0.2-31 ng/mL (QC tested).
Protocol
Western Blot验证
Detection of Anti c-Myc Tag Monoclonal Antibody (9E10) by Western Blot. Anti c-Myc Tag Monoclonal Antibody (9E10) at 0.02 μg/mL dilution + Serum Albumin,Human,His & Myc at 400 ng.
Secondary: (MFC)-Peroxidase AffiniPure GoatAnti-MouseIgG,FcγFragmentSpecific at 1/1500 dilution.
Predicted band size: 27 kDa | 50-55 kDa 12% Bis-Tris Protein Gel
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