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抗体来源（Source）
PE-Labeled Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09) is a monoclonal antibody recombinantly expressed from HEK293 cells, which provides higher batch consistency and long term security of supply.
应用（Application）
Flow Cytometry (Evaluation of CAR and SCFV containing 218 linker on Human cells).
克隆号（Clone）
P3C09
种属（Species）
Rabbit
亚型（Isotype）
Rabbit IgG | Rabbit Kappa
特异性（Specificity）
This product is a specific antibody specifically reacts with CAR or scFv containing the 218 linker sequence.
种属反应性（Reactivity）
Human
免疫原（Immunogen）
Purified 218 linker protein
偶联（Conjugate）
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
推荐稀释比（Recommended Dilution）
1:50
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 0.2% BSA, 0.03% Proclin300 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution.
- 2-8 °C for 12 months after reconstitution.
质量管理控制体系（QMS）
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数据展示
活性（Bioactivity）-FACS
Flow cytometric analysis of Anti-BCMA(C11D5.3) CAR 293 cells staining with PE-Labeled Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09) (Cat. No.218-PCFMY3) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL). PE signal was used to evaluate the binding activity (QC tested).
Protocol
Non-specificity of PE-Labeled Monoclonal Anti-Whitlow 218 Linker Antibody binding to CD3+ cells present in human PBMC. 5e5 of humanPBMCs were simultaneously stained with FITC anti-CD3 antibody and PE-Labeled Monoclonal Anti-Whitlow 218 Linker Antibody, RabbitIgG (P3C09) (Cat. No. 218-PCFMY3) (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both FITC and PE positive signals was used to evaluate the non-specific binding activity tohuman CD3+ cells (QC tested).
Protocol
Compared Data
Flow cytometric analysis of Anti-BCMA (C11D5.3) CAR 293 cells staining with PE-Labeled Monoclonal Anti-Whitlow 218 Linker Antibody, Rabbit IgG (P3C09) (Cat. No. 218-PCFMY3) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL) and Competitor C. PE signal was used to evaluate the binding activity. The biological activity level of 218-PCFMY3 is superior to Competitor C (Routinely tested).
Protocol
典型数据-Typical Data
Immunohistochemical analysis of paraffin embedded anti-BCMA CAR-T cells labelled with 218-PCFMY3.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with lHC staining protocol.
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