FGL1蛋白-肿瘤免疫治疗新通路

Immobilized Human LAG-3, Mouse IgG2a Fc Tag (Cat. No. LA3-H52Aa) at 10 μg/mL (100 μL/well) can bind Human FGL1, Fc Tag (Cat. No. FG1-H5258) with a linear range of 0.02-0.156 μg/mL.

Serial dilutions of Anti-LAG3, Neutralizing Antibody were added into Human FGL1, Fc Tag (Cat. No. FG1-H5258): Biotinylated Human LAG-3, Mouse IgG2a Fc,Avitag (Cat. No. LA3-H82F3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.42303 μg/mL.

Loaded Human LAG-3, Fc Tag (Cat. No. LA3-H5255) on Protein A Biosensor, can bind Human FGL1, His Tag (Cat. No. FG1-H52Hy) with an affinity constant of 7.09 nM as determined in BLI assay (ForteBio Octet Red96e).

FACS assay shows that Human FGL1, Fc Tag (Cat. No. FG1-H5258) can bind to 293T cells overexpressing human LAG3. The concentration of Human FGL1 is 3 μg/mL (Routinely tested).