Fc受体：抗体药半衰期评估与体外功能验证关键试剂

The purity of Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (Cat. No. FCM-C52W8) is more than 90% and the molecular weight of this protein is around 43-60 kDa verified by SEC-MALS.

Immobilized Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) on SA Chip can bind Herceptin® with an affinity constant of 0.267 μM as determined in a SPR assay (Biacore 8K) (QC tested).

Loaded Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-C5284 ) on SA Biosensor via Biotin his antibody, can bind Herceptin with an affinity constant of 0.13 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

The purity of Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (Cat. No. FCM-C52W8) is more than 90% and the molecular weight of this protein is around 43-60 kDa verified by SEC-MALS.

Immobilized Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) on SA Chip can bind Herceptin® with an affinity constant of 0.267 μM as determined in a SPR assay (Biacore 8K) (QC tested).

Loaded Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-C5284 ) on SA Biosensor via Biotin his antibody, can bind Herceptin with an affinity constant of 0.13 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

The purity of Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (Cat. No. FCM-C52W8) is more than 90% and the molecular weight of this protein is around 43-60 kDa verified by SEC-MALS.

Immobilized Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) on SA Chip can bind Herceptin® with an affinity constant of 0.267 μM as determined in a SPR assay (Biacore 8K) (QC tested).

Loaded Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-C5284 ) on SA Biosensor via Biotin his antibody, can bind Herceptin with an affinity constant of 0.13 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

The purity of Human CD32a (R167), His Tag (Cat. No. CDA-H5221) is more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.

Human CD64, His Tag (Cat. No. FCA-H52H1) captured on CM5 chip via anti-His antibody can bind Herceptin® with an affinity constant of 4.92 nM as determined in a SPR assay (Biacore 8K) (QC tested).

Loaded Human CD16a (V176), His Tag (Cat. No. CD8-H52H4) on HIS1K Biosensor, can bind Herceptin with an affinity constant of 0.447 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Batch consistency of Human Fc gamma RIIB / CD32b (Cat. No. CDB-H5228). The similarity of Human Fc gamma RIIB / CD32b (Cat. No. CDB-H5228) for different batches is more than 90%.

The purity of Human CD32a (R167), His Tag (Cat. No. CDA-H5221) is more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.

Human CD64, His Tag (Cat. No. FCA-H52H1) captured on CM5 chip via anti-His antibody can bind Herceptin® with an affinity constant of 4.92 nM as determined in a SPR assay (Biacore 8K) (QC tested).

Loaded Human CD16a (V176), His Tag (Cat. No. CD8-H52H4) on HIS1K Biosensor, can bind Herceptin with an affinity constant of 0.447 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Batch consistency of Human Fc gamma RIIB / CD32b (Cat. No. CDB-H5228). The similarity of Human Fc gamma RIIB / CD32b (Cat. No. CDB-H5228) for different batches is more than 90%.

The purity of Human CD32a (R167), His Tag (Cat. No. CDA-H5221) is more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.

Human CD64, His Tag (Cat. No. FCA-H52H1) captured on CM5 chip via anti-His antibody can bind Herceptin® with an affinity constant of 4.92 nM as determined in a SPR assay (Biacore 8K) (QC tested).

Loaded Human CD16a (V176), His Tag (Cat. No. CD8-H52H4) on HIS1K Biosensor, can bind Herceptin with an affinity constant of 0.447 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Batch consistency of Human Fc gamma RIIB / CD32b (Cat. No. CDB-H5228). The similarity of Human Fc gamma RIIB / CD32b (Cat. No. CDB-H5228) for different batches is more than 90%.

ADCC response to anti-human CD20 antibody (RLU). Anti-human CD20 antibody-induced ADCC activity was evaluated using Human CD16a (158V) (Luc) Jurkat Reporter Cell (Cat.No. SCJUR-STF067) in the presence of Raji cells that express CD20 endogenously. The EC50 of anti-human CD20 antibody was approximately 0.0028 μg/mL.

ADCP response to anti-human CD20 antibody (RLU). Anti-human CD20 antibody-induced ADCP activity was evaluated using Human CD32a (131H) (Luc) Jurkat Reporter Cell (Cat.No. SCJUR-STF069) in the presence of Raji cells that express CD20 endogenously. The EC50 was approximately 0.1054 μg/mL.

Human CD16a (158V) (Luc) Jurkat Reporter Cell Development Service (Cat.No. SCJUR-STF067)

Expression analysis of human CD16a (158V) on Human CD16a (158V) (Luc) Jurkat Reporter Cell by FACS. Human CD16a (158V) (Luc) Jurkat Reporter Cell or negative control cell were stained with PE-labeled anti-human CD16a antibody.

Human CD32a (131H) (Luc) Jurkat Reporter Cell Development Service (Cat.No. SCJUR-STF069)

Expression analysis of human CD32a on Human CD32a (131H) (Luc) Jurkat Reporter Cell by FACS. Cell surface staining was performed on Human CD32a (131H) (Luc) Jurkat Reporter Cell or negative control cell using PE-labeled anti-human CD32a antibody.

Human CD16a (158V) (Luc) Jurkat Reporter Cell Development Service (Cat.No. SCJUR-STF067)

Passage stability analysis by Signaling Bioassay. The continuously growing Human CD16a (158V) (Luc) Jurkat Reporter Cell was stimulated with serial dilutions of anti-human CD20 antibody in the presence of Raji cells that express CD20 endogenously. Anti-human CD20 antibody stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 14-26.

Human CD32a (131H) (Luc) Jurkat Reporter Cell Development Service (Cat.No. SCJUR-STF069)

Passage stability analysis by Signaling Bioassay. The continuously growing Human CD32a (131H) (Luc) Jurkat Reporter Cell was stimulated with serial dilutions of Anti-human CD20 antibody in the presence of Raji cells. Anti-human CD20 antibody stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 10-23.