抑制剂筛选试剂盒

使用PD-1 [Biotinylated] : PD-L1 抑制剂筛选试剂盒(Cat. No. EP-101)来检测抗PD-1中和抗体对PD-1: PD-L1结合的抑制效果

Anti-PD-1 neutralizing antibody was diluted from 10 μg/mL to 0.078 μg/ mL (69.628 nM to 0.544 nM) and loaded onto the plate coated by human PD-L1 in the presence of human PD-1-Biotin. Assay was performed according to the protocol in PD-1[Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

使用CD47: SIRP alpha [biotinylated]抑制剂筛选试剂盒(Cat. No. EP-102)来检测抗CD47中和性抗体对CD47: SIRP alpha结合的抑制效果

Serial dilutions of anti-CD47 neutralizing antibody (Catalog # CD7-NA002) (1:2 serial dilutions, from 10 μg/mL to 0.04 μg/mL or 66.67 nM to 0.26 nM) was added into CD47: biotinylated SIRP alpha binding reactions. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

使用IL-4 [biotinylated]: IL-4 R alpha抑制剂筛选试剂盒(Cat. No. EP-132) 来检测Human IL-4对IL-4: IL-4 R alpha结合的抑制效果

Serial dilutions of Human IL-4(Catalog # EP132-C03) (1:1 serial dilution, from 5 μg/mL to 0.01 μg/mL (295.86-0.58 nM)) was added into IL-4 R alpha: IL-4-Biotin binding reactions. The assay was performed according to the above-described protocol. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

使用Integrin α 4 β 7: MAdCAM-1 [Biotinylated]抑制剂筛选试剂盒(Cat. No. EP-155) 检测抗Integrin α 4 β 7中和抗体对Integrin α 4 β 7: MAdCAM-1结合的抑制效果

Serial dilutions of Anti-Integrin α 4 β 7 Neutralizing antibody (Catalog # EP155-C04) (1:1 serial dilution, from 5 μg/mL to 0.0098μg/mL) was added into Integrin α 4 β 7: Biotinylated MAdCAM-1 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).