CelThera™高品质培养基，成就细胞培养高光时刻

Human PBMCs were activated using 0.2 µg/mL GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (ACROBiosystems, Cat. No. GMP-MC0323) and 1 µg/mL GMP Monoclonal Anti-Human CD28 Antibody (ACROBiosystems, Cat. No. GMP-MC2824), cultured with CelThera™ GMP T Cell Expansion culture medium (ACROBiosystems, Cat. No. GMP-CM3101) supplemented with 500 IU/mL GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) for two weeks. The results showed that GMP human IL-2 protein, GMP monoclonal anti-human CD3 antibody (OKT3), GMP monoclonal anti-human CD28 antibody and CelThrea™ GMP T cell expansion medium could be used to culture T cells in a 3L large system. It can efficiently expand cells with high viability.

Cell ratio of the human various T cells. Human PBMCs were activated using 0.2 µg/mL GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (ACROBiosystems, Cat. No. GMP-MC0323) and 1 µg/mL GMP Monoclonal Anti-Human CD28 Antibody (ACROBiosystems, Cat. No. GMP-MC2824), and cultured in CelThera™ GMP T Cell Expansion culture medium (ACROBiosystems, Cat. No. GMP-CM3101) supplemented with 500 IU/mL GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) for two weeks. The result shows that percentage of CD3+ cells in living cells is above 99%. At the end of the culture process, the majority memory phenotype of T cells are TN/SCM, followed by TCM, which means that the cell harvest will have a promising effect, such as sustained proliferation, prolonged survival, and so on.

使用3E7冻存外周血来源PBMC种子细胞，20 mL 起始体积连续补液扩增， 13天扩增至3 L，收获超70亿细胞，第13天NK细胞纯度高于97%。整体培养周期更短

3E6冷冻保存的PBMC（外周血来源）种子细胞，以3mL的初始体积在底部带有透气膜的规模化静置培养反应器中进行连续饲养扩增。14天后，体积扩大到2000mL，收获了超过60亿个细胞，且收获时，γδT细胞纯度超过93%。