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ACROBiosystems专门搭建了基于HEK293表达系统的VLP技术平台，将构象完整的膜蛋白直接聚集在细胞表面，使这些复杂的膜蛋白转变为可溶性的、高浓度的蛋白用于抗体免疫和筛选，所制备的包膜VLPs在其固有的细胞膜上显示正确折叠的多次跨膜蛋白，从而能够诱导和筛选识别靶标天然构象的功能性抗体。
ACROBiosystems除提供VLP技术平台下常规生产的多次跨膜蛋白之外，同时也接收 定制服务。
多次跨膜蛋白的跨膜区域疏水性较高，在普通的缓冲液中很难保持正确的构象，可以通过添加去垢剂解决该问题。以昆虫细胞表达技术和哺乳动物细胞表达技术为基础，ACROBiosystems专门搭建了困难药物靶点跨膜蛋白的表达平台和纯化平台，并且已经从常用的去垢剂中筛选出包括Detergent（货号DC-11）在内的几种效果很好的去垢剂，大大增加多次跨膜靶点蛋白的水溶性，帮助其在溶液中形成天然正确的构象。
“Nanodisc”的磷脂层结构，是由膜支架蛋白(membrane scaffold proteins, MSPs)和磷脂分子构成的磷脂双分子层类膜结构，跨膜蛋白移除去垢剂后可以整合到Nanodisc的特殊结构中，保持其生物学活性并有很好的水溶性，应用更广泛。ACROBiosystems研发团队经过不断优化改进，已成功摸索出适用于工业化放大生产的Nanodisc组装工艺，实现了跨膜蛋白Nanodisc产品的长期稳定供货。
“Nanodisc-pro”技术平台采用创新的骨架原位组装策略，直接在跨膜蛋白的天然膜环境附近，利用骨架环绕包裹目标蛋白及其紧密相邻的天然磷脂，形成稳定的磷脂双分子层结构。该技术免除了传统方法中破坏性的去垢剂剥离与重组步骤，最大程度保留了跨膜蛋白的天然构象与活性。所获得的Nanodisc-pro产品不仅保持了跨膜蛋白的生物学功能，还具备优异的水溶性，应用广泛。
以下ELISA&SPR数据均可免费提供内部实验操作方法以供参考，点击申请Protocol即可获取。
Immobilized Human Claudin-18.2 Full Length Protein-VLP (Cat. No. CL2-H52P7) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 with a linear range of 0.2-3 ng/mL (QC tested).
Protocol
Human Claudin-18.2 Full Length Protein-VLP (Cat. No. CL2-H52P7) captured on CM5 Chip via Anti-Claudin-18.2 antibody can bind Anti-Claudin-18.2 antibody with an affinity constant of 0.374 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
2e5 of Anti-Claudin-18.2 CAR-293 cells were stained with 100 μL of 3 μg/mL of Fluorescent Human Claudin-18.2 Full Length Protein-VLP (Cat. No.CL2-HF218) and negative control protein respectively, FITC signals was used to evaluate the binding activity (QC tested).
Protocol
Immobilized Rituximab at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD20 Full Length, His,Avitag (Cat. No. CD0-H82E5) with a linear range of 4-63 ng/mL (in presence of DDM and CHS) (QC tested).
Protocol
Biotinylated Human CD20 Full Length, His,Avitag (Cat. No. CD0-H82E5) captured on Biotin CAP-Series S Sensor Chip can bind Rituximab with an affinity constant of 1.73 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore T200) (QC tested).
Human CD133 Full Length, His Tag (Nanodisc) （(Cat. No. CD3-H52H1)）on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Flow cytometric analysis of overexpressing Anti-Claudin-6(BBz) CAR-293 cells staining with Fluorescent Human Claudin-6 Full Length Protein, Flag,His Tag, GFP Tag (Cat. No. CL6-H52M3) (2e5 of Anti-Claudin-6(BBz) CAR-293 cells were stained with 100 μL of 5μg/mL of Fluorescent Human Claudin-6 Full Length Protein, Flag,His Tag,GFP Tag), compared with negative control protein analyzed with FACS (QC tested).
Protocol
Immobilized Fluorescent Human Claudin-6 Full Length Protein, Flag,His Tag,GFP Tag (Cat. No. CL6-H52M3) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human Claudin 6 antibody, Human IgG1 with a linear range of 0.05-2 ng/mL (QC tested).
Protocol
Anti-Claudin-6 Antibody, Human IgG1 captured on Protein A Chip can bind Fluorescent Human Claudin-6 Full Length Protein, Flag,His Tag,GFP Tag (Cat. No. CL6-H52M3) with an affinity constant of 1.52 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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