Recombinant Proteins iso9001 ISO13485
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Human IL-1 beta / IL-1F2 Protein, Tag Free (MALS verified)

分子别名(Synonym)

IL1B,IL-1BETA,IL1F2,IL-1β

表达区间及表达系统(Source)

Human IL-1 beta, Tag Free (ILB-H4110) is expressed from E.coli cells. It contains AA Ala 117 - Ser 269 (Accession # P01584-1).

Predicted N-terminus: Met

蛋白结构(Molecular Characterization)

Online(Ala 117 - Ser 269) P01584-1

This protein carries no "tag".

The protein has a calculated MW of 17.5 kDa. The protein migrates as 17 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human IL-1 beta, Tag Free (Cat. No. ILB-H4110) SDS-PAGE gel

Human IL-1 beta, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human IL-1 beta, Tag Free (Cat. No. ILB-H4110) MALS images

The purity of Human IL-1 beta, Tag Free (Cat. No. ILB-H4110) was more than 95% and the molecular weight of this protein is around 15-23 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human IL-1 beta, Tag Free (Cat. No. ILB-H4110) at 10 μg/mL (100 μL/well) can bind Human IL-1 RII, Fc Tag (Cat. No. IL2-H4256) with a linear range of 8-63 ng/mL (QC tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human IL-1 RII, Fc Tag (Cat. No. IL2-H4256) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL-1 beta, Tag Free (Cat. No. ILB-H4110) with an affinity constant of 0.622 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-Cell based assay

Biotinylated Human CELL_BASE

Human IL-1 beta, Tag Free (Cat. No. ILB-H4110) stimulates secretion of IFN-gamma by NK-92 human natural killer lymphoma cells. The EC50 for this effect is 1.02-1.33 ng/mL (Routinely tested).

Protocol

 

背景(Background)

Interleukin-1 beta (IL-1β) is also known as catabolin, is a cytokine protein that in humans is encoded by the IL1B gene. IL-1β precursor is cleaved by caspase 1 (interleukin 1 beta convertase). Cytosolic thiol protease cleaves the product to form mature IL-1 beta. IL1β are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL1α and IL1β are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1β is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

 

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IL-1 beta靶点信息
英文全称:Interleukin-1 beta
中文全称:白细胞介素-1β
种类:Homo sapiens
上市药物数量:4详情
临床药物数量:7详情
最高研发阶段:批准上市
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