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Monoclonal Anti-DNP antibody, Human IgG1 (N297A) Isotype Control (carrier free, MALS verified)

表达区间及表达系统(Source)

Dinitrophenyl (DNP) is a hapten that is not normally expressed in the target tissue. Therefore, anti-DNP antibody has no relevant specificity to a target antigen, and can be used as isotype control antibody to differentiate non-specific background signal from specific antibody signal in various in vitro and in vivo studies. Monoclonal Anti-DNP antibody, Human IgG1 (N297A) Isotype Control was purified from HEK293 cell culture.

亚型(Isotype)

Human IgG1 (N297A)/kappa

特异性(Specificity)

应用说明(Application)

This antibody is suitable for use as a non-targeting isotype control in various in vitro and in vivo studies. It can also be used as a negative control in various applications such as ELISA, Western blot, immunofluorescence, immunohistochemistry, immunoprecipitation, and flow cytometry. Each laboratory should determine an optimum working titer for use in its particular application.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

内毒素(Endotoxin)

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

存储(Storage)

Avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Monoclonal Anti-DNP antibody, Human IgG1 (N297A) Isotype Control (Cat. No. DNP-MB273) SDS-PAGE gel

Monoclonal Anti-DNP antibody, Human IgG1 (N297A) Isotype Control on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Monoclonal Anti-DNP antibody, Human IgG1 (N297A) Isotype Control (Cat. No. DNP-MB273) MALS images

The purity of Monoclonal Anti-DNP antibody, Human IgG1 (N297A) Isotype Control(Cat. No. DNP-MB273) is more than 90% and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Monoclonal Anti-DNP antibody, Human IgG1 (N297A) Isotype Control (Cat. No. DNP-MB273) with a linear range of 1-10 ng/mL (QC tested).

Protocol

背景(Background)

A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.

 

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