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Monoclonal Anti-DNP antibody, Human IgG1 Isotype Control

Basic Information

Species Reactivity Human
Host Cells HEK293 cells
MW 50 and 25 kDa for the heavy and light chains, respectively (150 kDa)
Isotype Human IgG1, Kappa LC
Clonality Monoclonal

Description

Dinitrophenyl (DNP) is a hapten that is not normally expressed in the target tissue. Therefore, anti-DNP antibody has no relevant specificity to a target antigen, and can be used as isotype control antibody to differentiate non-specific background signal from specific antibody signal in various in vitro and in vivo studies.

Monoclonal Anti-DNP (Anti-Hapten) antibody, Human IgG1 was purified from HEK293 cell culture at ACRObiosystems, and specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.

Purification

Protein A affinity chromatography from HEK293 supernatants.

Format

Lyophilized Powder

Application

This antibody is suitable for use as a non-targeting isotype control in various in vitro and in vivo studies. It can also be used as a negative control in various applications such as ELISA, Western blot, immunofluorescence, immunohistochemistry, immunoprecipitation, and flow cytometry. Each laboratory should determine an optimum working titer for use in its particular application.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally mannitol or trehalose are added as protectants before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state.
-70 ℃ for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)


 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Monoclonal Anti-DNP antibody, Human IgG1 Isotype Control (Cat. No. DNP-M2) with a linear range of 2-20 ng/mL (QC tested).

Protocol

文献引用(Citations)

 

Background

A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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