Recombinant Proteins iso9001 ISO13485
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Human CD58 / LFA-3 Protein, Fc Tag

分子别名(Synonym)

CD58,LFA3,Ag3

表达区间及表达系统(Source)

Human CD58, Fc Tag (LF3-H5256) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Arg 215 (Accession # AAH05930).

Predicted N-terminus: Phe 29

蛋白结构(Molecular Characterization)

Online(Phe 29  - Arg 215) AAH05930

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 48.1 kDa. As a result of different glycosylation, the protein migrates as 60-75 kDa under reducing (R) condition, and 125-150 kDa under non-reducing (NR) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CD58, Fc Tag (Cat. No. LF3-H5256) SDS-PAGE gel

Human CD58, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CD2, His Tag (Cat. No. CD2-H5226) at 2 μg/mL (100 μL/well) can bind Human CD58, Fc Tag (Cat. No. LF3-H5256) with a linear range of 10-78 ng/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded bind Human CD58, Fc Tag (Cat. No. LF3-H5256) on Protein A Biosensor, can bind Human CD2, His Tag (Cat. No. CD2-H5226) with an affinity constant of 8.42 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

CD antigen CD58 is also known as Lymphocyte function-associated antigen 3 (LFA-3). CD58 is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. CD58 /LFA3 is ligand of the T-lymphocyte CD2 glycoprotein, which binds to CD2 (LFA-2) [3] on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. Polymorphisms in the CD58 gene are associated with increased risk for multiple sclerosis.

 

前沿进展

 
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CD58靶点信息
英文全称:Lymphocyte function-associated antigen 3
中文全称:淋巴球功能抗原3型 
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床阶段不明
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