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 >  Protein>CD155 >CD5-H5254

Human CD155 / PVR Protein, Mouse IgG2a Fc Tag, low endotoxin

分子别名(Synonym)

PVR,FLJ25946,PVS,CD155,TAGE4,HVED,NECL5

表达区间及表达系统(Source)

Human CD155, Mouse IgG2a Fc Tag (CD5-H5254) is expressed from human 293 cells (HEK293). It contains AA Trp 21 - Asn 343 (Accession # NP_006496.4).

Predicted N-terminus: Trp 21

Request for sequence

蛋白结构(Molecular Characterization)

CD155 Structure

This protein carries a mouse IgG2a Fc tag at the C-terminus

The protein has a calculated MW of 62.2 kDa. The protein migrates as 70-100 kDa under reducing (R) condition, and 130-160 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

CD155 SDS-PAGE

Human CD155, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

CD155 ELISA

Immobilized Human TIGIT, Fc Tag (Cat. No. TIT-H5254) at 2 μg/mL (100 μL/well) can bind Human CD155, Mouse IgG2a Fc Tag (Cat. No. CD5-H5254) with a linear range of 0.3-2 ng/mL (QC tested).

Protocol

CD155 ELISA

Immobilized Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) at 2 μg/mL (100 μL/well) can bind Human CD155, Mouse IgG2a Fc Tag (Cat. No. CD5-H5254) with a linear range of 1-16 ng/mL (Routinely tested).

Protocol

CD155 ELISA

Serial dilutions of Human TIGIT Neutralizing antibody were added into Human CD155, Mouse IgG2a Fc Tag (Cat. No. CD5-H5254): Biotinylated Human TIGIT, Fc,Avitag (Cat. No. TIT-H82F1) binding reactions. The half maximal inhibitory concentration (IC50) is 0.08116 μg/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

CD155 BLI

Loaded Human CD155, Mouse IgG2a Fc Tag (Cat. No. CD5-H5254) on Protein A Biosensor, can bind Human TIGIT, His Tag with an affinity constant of 0.24 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

CD155 BLI

Loaded Human CD155, Mouse IgG2a Fc Tag (Cat. No. CD5-H5254) on Protein A Biosensor, can bind Human DNAM-1, His Tag (Cat. No. DN1-H52H6) with an affinity constant of 0.57 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

活性(Bioactivity)-FACS

CD155 FACS

FACS assay shows that Human CD155, Mouse IgG2a Fc Tag (Cat. No. CD5-H5254) can bind to 293T cell overexpressing human TIGIT. The concentration of CD155 is 1 μg/mL (Routinely tested).

Protocol

CD155 FACS

FACS analysis shows that the binding of Human CD155, Mouse IgG2a Fc Tag (Cat. No. CD5-H5254) to 293T overexpressing TIGIT was inhibited by increasing concentration of neutralizing Anti-Human TIGIT MAb. The concentration of CD155 used is 1 μg/mL. The IC50 is 0.1413 μg/mL (Routinely tested).

Protocol

 

背景(Background)

CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells.
CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells. The fulllength (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N terminal V type and two C2 type Ig like domains.
CD155 is a transmembrane protein with 3 extracellular immunoglobulin-like domains, D1-D3, where D1 is recognized by the virus. Low resolution structures of CD155 complexed with poliovirus have been obtained using electron microscopy while a high resolution structures of theectodomain D1 and D2 of CD155 were solved by x-ray crystallography.

 

前沿进展

 
 
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CD155靶点信息
英文全称:CD155 Antigen
中文全称:CD155抗原
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床二期
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