Recombinant Proteins iso9001 ISO13485
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Biotinylated Human IL-17 RA / CD217 Protein, Fc,Avitag™

分子别名(Synonym)

CD217,CDw217,IL-17RA,IL17R,CANDF5,hIL-17R

表达区间及表达系统(Source)

Biotinylated Human IL-17 RA, Fc,Avitag (ILA-H82F1) is expressed from human 293 cells (HEK293). It contains AA Leu 33 - Trp 320 (Accession # Q96F46-1).

Predicted N-terminus: Leu 33

蛋白结构(Molecular Characterization)

Online(Leu 33 - Trp 320) Q96F46-1

This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

The protein has a calculated MW of 61.9 kDa. As a result of glycosylation, the protein migrates as 80-115 kDa under reducing (R) condition, and 150-200 kDa under non-reducing (NR) condition (SDS-PAGE).

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human IL-17 RA, Fc,Avitag (Cat. No. ILA-H82F1) SDS-PAGE gel

Biotinylated Human IL-17 RA, Fc,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human IL17A, Tag Free (Cat. No. ILA-H5118) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-17 RA, Fc,Avitag (Cat. No. ILA-H82F1) with a linear range of 2-31 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human IL-17F (H161R), His Tag (Cat. No. ILF-H4240) at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-17 RA, Fc,Avitag (Cat. No. ILA-H82F1) with a linear range of 0.02-0.313 μg/mL (Routinely tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Human IL-17 RA, Fc,Avitag (Cat. No. ILA-H82F1) on SA Biosensor, can bind Human IL17A, Tag Free (Cat. No. ILA-H5118) with an affinity constant of 1.29 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Interleukin 17 receptor A (IL17RA) is also known as cluster of differentiation w217 (CDw217), is a pro-inflammatory cytokine secreted by activated T-lymphocytes, belong to ubiquitous type I membrane glycoprotein, and binds with low affinity to interleukin 17A (IL17A). IL 17R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested . IL 17RA associates with IL 17RC to form a signaling receptor complex for IL 17 and IL 17F . Ligand and IL 17RA ligation promotes T cell activation and the production of IL - 6, G-CSF, SCF, and multiple pro-inflammatory chemokines. Defects in IL17RA are the cause of familial candidiasis type 5 (CANDF5).

 

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IL-17 RA靶点信息
英文全称:Interleukin-17 receptor A
中文全称:白细胞介素-17受体A亚基
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:2详情
最高研发阶段:批准上市
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