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Biotinylated Human BCMA / TNFRSF17 Protein, Fc,Avitag™ DMF Filed

分子别名(Synonym)

TNFRSF17,CD269,BCM,BCMA

表达区间及表达系统(Source)

Biotinylated Human BCMA, Fc,Avitag (BC7-H82F0) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54 (Accession # NP_001183.2).

Predicted N-terminus: Met 1

蛋白结构(Molecular Characterization)

Online(Met 1 - Ala 54) NP_001183.2

This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

The protein has a calculated MW of 33.8 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human BCMA, Fc,Avitag (Cat. No. BC7-H82F0) SDS-PAGE gel

Biotinylated Human BCMA, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

获取HPLC数据

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) at 2 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, Fc,Avitag (Cat. No. BC7-H82F0) with a linear range of 0.1-2 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Anti-Human BCMA Mab at 2 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, Fc,Avitag (Cat. No. BC7-H82F0) with a linear range of 0.1-3 ng/mL (Routinely tested).

Protocol

Biotinylated Human ELISA

Quantitative Analysis of Bispecific T-cell Engager (CD3 X BCMA) in human serum.
Immobilized Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-H52W1) at 2 μg/mL, add increasing concentrations of Bispecific T cell Engager (CD3 X BCMA) in 10% human serum and then add Biotinylated BCMA Fc,Avitag (Cat. No. BC7-H82F0) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Routinely tested).

Protocol

Biotinylated Human ELISA

Immobilized Human APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) at 1 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, Fc,Avitag (Cat. No. BC7-H82F0) with a linear range of 0.1-2 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-FACS

Biotinylated Human FACS

2e5 of anti-BCMA CAR-293 cells were stained with 100 μL of 3 μg/mL of Biotinylated Human BCMA, Fc,Avitag (Cat. No. BC7-H82F0) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).

Protocol

 

CAR阳性表达率检测(Evaluation of CAR expression )

FACS analysis of anti-BCMA CAR expression

CAR

Human T cells were transfected with anti-BCMA CAR and cultured for 3 days. Three days post-transfection, 1e6 cells were first incubated with 50 μL Biotinylated Human BCMA, Fc,Avitag (Cat. No. BC7-H82F0, 8 μg/mL), washed and then stained with PE Streptavidin and analyzed by flow cytometry. (Data are kindly provided by PREGENE Biopharma)

Protocol

 

文献引用(Citations)

背景(Background)

Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.

 

前沿进展

 
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BCMA靶点信息
英文全称:B-cell maturation protein
中文全称:B细胞成熟蛋白
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:42详情
最高研发阶段:批准上市
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