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 >  经HPLC验证的蛋白>BCMA >BCA-H522y

Human BCMA / TNFRSF17 Protein,His Tag, premium grade DMF Filed

分子别名(Synonym)

TNFRSF17,CD269,BCM,BCMA

表达区间及表达系统(Source)

Human BCMA, His Tag, premium grade (BCA-H522y) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54 (Accession # Q02223-1 ).

Predicted N-terminus: Met 1

It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

Request for sequence

蛋白结构(Molecular Characterization)

BCMA Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 7.8 kDa. The protein migrates as 12 kDa and 14-18 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

无菌(Sterility)

The sterility testing was performed by membrane filtration method.

支原体(Mycoplasma)

Negative.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 24 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

BCMA SDS-PAGE

Human BCMA, His Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-HPLC

BCMA SEC-HPLC

The purity of Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) was greater than 80% as determined by SEC-HPLC.

 

活性(Bioactivity)-ELISA

BCMA ELISA

Immobilized Human BAFF, Fc Tag (Cat. No. BAF-H4268) at 5 μg/mL (100 μL/well) can bind Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) with a linear range of 0.031-0.5 μg/mL (QC tested).

Protocol

BCMA ELISA

Immobilized Human APRIL, Fc Tag (Cat. No. APL-H5267) at 5 μg/mL (100 μL/well) can bind Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) with a linear range of 6-25 ng/mL (Routinely tested).

Protocol

BCMA ELISA

Immobilized Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human BCMA Antibody, Human IgG1 with a linear range of 0.2-3 ng/mL (Routinely tested).

Protocol

BCMA ELISA

Immobilized Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) at 2 μg/mL (100 μL/well) can bind Anti-BCMA Neutralizing Antibody, Mouse IgG1 with a linear range of 0.2-3 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-SPR

BCMA SPR

Anti-Human BCMA MAb (human IgG1) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) with an affinity constant of 13.0 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

BCMA SPR

Anti-BCMA Neutralizing Antibody, Mouse IgG1 captured on CM5 chip via anti-mouse antibodies surface can bind Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) with an affinity constant of 0.234 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

BCMA BLI

Loaded Anti-Human BCMA MAb (human IgG1) on Protein A Biosensor, can bind Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) with an affinity constant of 29.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

BCMA BLI

Loaded Human APRIL, Fc Tag (Cat. No. APL-H5267) on Protein A Biosensor, can bind Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) with an affinity constant of 10.9 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

BCMA BLI

Loaded Human BAFF, Fc Tag (Cat. No. BAF-H4268) on Protein A Biosensor, can bind Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) with an affinity constant of 0.64 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

活性(Bioactivity)-FACS

BCMA FACS

2e5 of BCMA-CAR-293 cells transfected with anti-BCMA-scFv were stained with 100 μL of 0.3 μg/mL of Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) and negative control protein respectively, washed and then followed by FITC anti-6xHis tag antibody and analyzed with FACS (Routinely tested).

Protocol

背景(Background)

Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.

 

前沿进展

 
 
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BCMA靶点信息
英文全称:B-cell maturation protein
中文全称:B细胞成熟蛋白
种类:Homo sapiens
上市药物数量:2详情
临床药物数量:58详情
最高研发阶段:批准上市
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