分子别名（Synonym）
TNFRSF17,CD269,BCM,BCMA
表达区间及表达系统（Source）
Human BCMA Protein, His Tag (HPLC-verified) (BCA-H522y) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54 (Accession # Q02223-1).
Predicted N-terminus: Met 1
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 7.8 kDa. The protein migrates as 13-19 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
无菌（Sterility）
The sterility testing was performed by membrane filtration method described in USP <71> and Eur. Ph. 2.6.1.
纯度（Purity）
>90% as determined by SDS-PAGE.
>80% as determined by SEC-HPLC.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human BCMA Protein, His Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-HPLC
The purity of Human BCMA Protein, His Tag (HPLC-verified) was greater than 80% as determined by SEC-HPLC.
活性（Bioactivity）-ELISA
Immobilized Human BAFF, Fc Tag (Cat. No. BAF-H4268) at 5 μg/mL (100 μL/well) can bind Human BCMA Protein, His Tag (Cat. No. BCA-H522y) with a linear range of 0.031-0.5 μg/mL (QC tested).
Protocol
Immobilized Human APRIL, Fc Tag (Cat. No. APL-H5267) at 5 μg/mL (100 μL/well) can bind Human BCMA Protein, His Tag (Cat. No. BCA-H522y) with a linear range of 6-25 ng/mL (Routinely tested).
Protocol
Immobilized Human BCMA Protein, His Tag (Cat. No. BCA-H522y) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human BCMA Antibody, Human IgG1 with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Human BCMA Protein, His Tag (Cat. No. BCA-H522y) at 2 μg/mL (100 μL/well) can bind Anti-BCMA Neutralizing Antibody, Mouse IgG1 with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Anti-Human BCMA MAb (human IgG1) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with an affinity constant of 13.0 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Anti-BCMA Neutralizing Antibody, Mouse IgG1 captured on CM5 chip via anti-mouse antibodies surface can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with an affinity constant of 0.234 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Anti-Human BCMA MAb (human IgG1) on Protein A Biosensor, can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with an affinity constant of 29.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human APRIL, Fc Tag (Cat. No. APL-H5267) on Protein A Biosensor, can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with an affinity constant of 10.9 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human BAFF, Fc Tag (Cat. No. BAF-H4268) on Protein A Biosensor, can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with an affinity constant of 0.64 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
活性（Bioactivity）-FACS
2e5 of BCMA-CAR-293 cells transfected with anti-BCMA-scFv were stained with 100 μL of 0.3 μg/mL of Human BCMA / TNFRSF17 Protein,His Tag DMF Filed (Cat. No. BCA-H522y) and negative control protein respectively, washed and then followed by FITC anti-6xHis tag antibody and analyzed with FACS. (Routinely tested).
Protocol
文献引用（Citations）
FULLY HUMANIZED ANTI-B CELL MATURATION ANTIGEN (BCMA) SINGLE-CHAIN ANTIBODY AND USE THEREOF
Authors: Li Zhiyuan, Zhai Tianhang, et al.
Journal: US20200339699A1 2020
Application: BLI
背景（Background）
Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.
