FcRn,FCGRT & B2M
MABSol® Biotinylated Rat FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (FCM-R82W7) is expressed from human HEK293 cells. It contains AA Ala 23 - Ser 298 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P13599-1(FCGRT) & P07151-1(B2M)).
Predicted N-terminus: Ala 23 (FCGRT) & Ile 21 (B2M)
Request for sequence
Biotinylated Rat FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.7 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag and subunit Beta-2 microglobulin (B2M) is fused with a Strep II tag at the C-terminus. The reducing (R) protein migrates as 45-50 kDa (FCGRT) and 14 kDa (B2M) respectively due to different glycosylation.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the HABA assay / binding ELISA.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Rat FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Rat FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-R82W7) is more than 90% and the molecular weight of this protein is around 53-71 kDa verified by SEC-MALS.
Biotinylated Rat FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-R82W7) captured on Biotin CAP-Series S Sensor Chip can bind Herceptin® with an affinity constant of 10.3 nM as determined in a SPR assay (Biacore T200) (QC tested).
FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.